止喘灵口服液治疗支气管哮喘的药效学研究

Experimental study of zhi-chuan-ling oral liquor on asthma

目的 :根据止喘灵口服液宣肺定喘之功效 ,探讨其止喘的药理作用和机制。方法 :采用喷雾致喘法制备哮喘模型 ,观察不同剂量止喘灵口服液的引喘潜伏期 ;体外实验观察止喘灵口服液对豚鼠离体气管和回肠平滑肌的收缩以及气管容积和肺支气管灌流量的影响。结果 :止喘灵口服液能显著延长乙酰胆碱组胺混合刺激所致豚鼠哮喘的引喘潜伏期 ,对抗乙酰胆碱或组胺对豚鼠离体支气管或回肠平滑肌有收缩作用 ,并可增加气管容积和离体肺支气管灌流量。结论 :止喘灵口服液对胆碱能神经递质乙酰胆碱或肥大细胞炎性介质组胺所致的气道高反应性和通气障碍都有明显的对抗作用 ,缓解支气管平滑肌痉挛 ,改善肺通气功能 ,从而达到止咳平喘的效果。

AIM: To evaluate its pharmacodynamic effects of zhi-chuan-ling (ZCL) oral liquor, a traditional Chinese medicinal prescription, on asthma. METHODS: Asthma models of guinea pigs were established by aerosol inhalation of mixed solution of histamine and acetylcholine for the observation of effects of ZCL on the latent period of asthma attack. Some other indices were also investigated including the contraction of smooth muscle strips isolated from the trachea or intestinum ileum, and variations of the tracheal volume and the bronchotracheal perfusion in guinea pigs. RESULTS: ZCL elongated the latent period of asthma attack induced by areol inhalation of mixed solution of histamine and acetylcholine, antagonized activities against the contraction of the trachea and intestinum ileum induced by histamine or acetlcholine, and increased the tracheal volume and the bronchotracheal perfusion in ginea pigs in vitro. CONCLUTION: ZCL can reduce airway hyperresponsibility, relax bronchospasm, remit ventilation disturbance, and improve ventilatory function by its biologic activities against acetlcholin and histamine.