利用非侵入性光学成像技术监测在体肿瘤的生长

In vivo Optical Imaging of the Tumor Growth

利用电穿孔技术将gfp表达质粒转染于SP2/0细胞,通过G418筛选获得稳定表达GFP的小鼠骨髓瘤细胞株SP2/0-GFP细胞。将稳定的转化细胞移植于同系BALB/c小鼠后腿、耳部皮下及腹腔成瘤,每隔一天对小鼠肿瘤成像,跟踪肿瘤的生长过程。结果表明,探测到的GFP荧光强度和发光范围与肿瘤的生长或消亡相关,从而证明可以利用GFP作为肿瘤细胞的标记,结合光学成像技术,对肿瘤生长过程实时追踪。

The myeloma cell line, SP2/0 from the BALB/c mouse, was engineered to stably express highlevels of the green fluorescent protein (GFP) by electroporation technology and selected with G418. The GFPexpressing tumor cells(SP2/0-GFP) were implanted into BALB/c mouse, and optical images visualized realtime tumor growth. The results show that the fluorescence of GFP is comparably with the tumor growth and/or depress. The GFP imaging technology presented in this report will facilitate studies of tumor growth.