摘要
Objective: To study the molecular mechanism ofdifferent tumorigenicity in nude mice of human leukemiacell lines K562-n and K562. Methods: To analyze the genes differently expressed between K562 and K562-n cells byusing cDNA microarray technique. Results: Among the12800 genes detected, some genes involved in materialmetabolism and material transport were differentlyexpressed between K562-n and K562 cells. These genesinclude homo sapiens placenta-specific ATP-binding cassette transporter gene, dihydrodiol dehydrogenase gene, hepatic dihydrodiol dehydrogenase gene, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase,lysophosphatidic acid acyltransferase, alpha gene,argininosuccinate lyase gene, mitochondrial isocitrtatedehydrogenase, adhesion protein SQM1 gene, dimethylarginine dimethylamino-hydrolase gene, M1subunit of ribonucleotide reductase and farnesylpyrophosphate synthetase gene. Conclusion: The hightumorigenicity of K562-n cells is related to the differentexpression of some genes concerned with cell metabolismand material transpoert.
Objective: To study the molecular mechanism ofdifferent tumorigenicity in nude mice of human leukemiacell lines K562-n and K562. Methods: To analyze the genes differently expressed between K562 and K562-n cells byusing cDNA microarray technique. Results: Among the12800 genes detected, some genes involved in materialmetabolism and material transport were differentlyexpressed between K562-n and K562 cells. These genesinclude homo sapiens placenta-specific ATP-binding cassette transporter gene, dihydrodiol dehydrogenase gene, hepatic dihydrodiol dehydrogenase gene, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase,lysophosphatidic acid acyltransferase, alpha gene,argininosuccinate lyase gene, mitochondrial isocitrtatedehydrogenase, adhesion protein SQM1 gene, dimethylarginine dimethylamino-hydrolase gene, M1subunit of ribonucleotide reductase and farnesylpyrophosphate synthetase gene. Conclusion: The hightumorigenicity of K562-n cells is related to the differentexpression of some genes concerned with cell metabolismand material transpoert.
基金
This work was supported by the National Natural Science Foundation of China (No. 39770330).
