用表面等离子体子共振传感器测定人心肌肌钙蛋白I的研究

An Optical Biosensor Based on Surface Plasmon Resonance for Determination of Human Cardiac Troponin I

采用自组装表面等离子体子共振 (SPR)传感装置 ,固定入射角 ,以波长为变量 ,以 CCD为检测系统 ,用对金和抗体均有较强吸附作用的葡萄球菌 A蛋白作为基底膜 ,观测了人心肌肌钙蛋白 I的抗体和抗原之间免疫反应的动力学过程 ,并进行了人心肌肌钙蛋白 I的定量测定 .结果表明 ,人心肌肌钙蛋白 I的浓度在5 .0～ 5 0 μg/L范围内与传感器的响应值呈线性关系 .

A novel optical biosensor based on simultaneous multi-wavelength detection surface plasmon resonance(SPR) was developed for diagnosing acute myocardial infaction. The sensor was designed on the basis of fixing the angle of incidence and measuring the reflected intensities in the wavelength range of 400-800 nm. The SPR spectra are shown in terms of reflected light intensity verus wavelength of incident light. The intensity of the reflected light is the minimum at the resonant wavelength. On the sensing element of a right angle the glass prism is a thin(50 nm) metallic gold film coated by vacuum-deposition. Molecular self-assembling in solution is used to form the sensing membrane on the gold substrate. Human cardiac troponin I was determined in the concentration range of 5.0-50 μg/L. Under the selected experimental conditions, the sensor has a good sensitivity, repeatability, reversibility and selectivity.