蜂毒素的SDS-PAGE质控方法研究

Study on quality control of melittin by SDS-PAGE method

目的 建立以 SDS- PAGE进行蜂毒素质量控制的方法。方法 将通过凝胶色谱法从蜜蜂毒中分离纯化所得到的蜂毒素 ,以 SDS- PAGE法测定其相对分子质量 ,并通过凝胶成像扫描测定蜂毒素纯度。结果 蜂毒素样品电泳后在凝胶块上显示为均一条带 ,相对分子质量约为 2 80 0 ,含量为 (90 .5 9± 0 .85 ) % ,与 HPL C测定结果平行。结论 SDS- PAGE法作为蜂毒素的质控方法 ,结果准确、可靠。

Object To establish a SDS PAGE method on quality control of melittin. Methods Melittin was extracted and purified from bee venom by gel chromatography. Purity and molecular mass of melittin were determined by SDS PAGE. Results In the gelatinous board, the sample was a single peptide chain with a molecular mass of about 2 800 dalton as revealed by SDS PAGE. The purity was (90 59±0 85)%, which was in parallel with the result tested by HPLC. Conclusion SDS PAGE is accurate, reliable and simple for the determination of melittin.