摘要
目的 :研究环氧化合物 11,12 epoxyeicosatrienoicacid (11,12 EET)对未成熟兔离体心脏缺血再灌注损伤的保护作用及机制。 方法 :16只未成熟兔构成随机分组样本 ,分成对照组 (St ThomasNo 2停搏液处理 )和实验组 (St ThomasNo 2停搏液 + 11,12 EET处理 )各 8只。利用非循环式Langendorff灌注装置 ,测定心脏停搏 2h (15℃ )和再灌注 1h (3 7℃ )的左心室变化压、舒张末期压、最大压力变化速率、心肌含水量和冠状动脉流量、心肌钙离子含量以及心肌酶肌酸激酶、乳酸脱氢酶改变等。 结果 :实验组的心功能恢复、心肌水肿程度和心肌酶改变均优于对照组。 结论 :St ThomasNo 2停搏液中加入 11,12 EET可增强其对未成熟兔心缺血再灌注损伤的保护效果。
Objective: This study was designed to determine the effects of 11,12-epoxyeicosatrienoic acid (11,12-EET) in protecting myocardium of immature rabbit hearts injured by ischemic reperfusion. Methods: Isolated rabbit hearts were randomly assigned to either group 1 (St.Thomas No.2 solution, n =8) or group 2 (St.Thomas No.2 solution plus 11,12-EET, n =8).By means of Langendorff technique, these isolated rabbit hearts underwent 2 hours of ischemia under hypothermia (15℃), and then 1 hour of reperfusion (37℃). We measured the preischemia and postischemia indexes of left ventricular developed pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), ±dp/pt max , myocardial water content and coronary blood effluxent (CBE). The myocardial calcium content and the values of creatine kinase and lactate dehydrogenase were also observed. Results: Postischemic recovery of myocardial function and the improved myocardial edema were significantly better in group 2. There was also little change of creatine kinase in group 2. Conclusions: These data suggest that 11,12-EET in addition to the cardioplegic solution has better myocardial protective effect.
出处
《中国循环杂志》
CSCD
北大核心
2003年第3期224-226,共3页
Chinese Circulation Journal
基金
中国医学科学院重点基金项目 (0 2 1 98)
