功能高分子磁性微球的制备及分析应用

Preparation of the Polymer-Coated Magnetic Microsphere Modified with Carboxyl Group and Its Analytical Application

通过分散聚合法制备功能高分子磁性微球 ,并有效地控制微球的粒径范围和微球表面活性基团。以乙醇 水为分散体系 ,对磁流体Fe3O4 表面进行苯乙烯和丙烯酸共聚反应 ,制得粒径均匀、分散性好、带有羧基的磁性微球。该微球经活化后和抗癌药物阿霉素结合 ,在自制磁性电解池中 ,可用金膜电极进行电化学检测。阿霉素磁性微球在 - 0 .4 0V处有一个明显的还原峰 ,在浓度为 7.2 5× 10 - 1 0 ～ 7.2 5× 10 - 9mol L范围内峰电流和阿霉素浓度的关系呈线性 ,检测下限达 3.6× 10 - 1 0 mol L。

The dispersing polymerization method was used to prepare the magnetic microsphere (MMS) with designed size and active function group. The magnetic nuclei of Fe3O4 were copolymerized with styrene and acrylic acid to form the polymer MMS modified with carboxyl group. The resulted MMS was uniform and dispersible. Adriamycin, an anti-tumor drug was coupled to the activated MMS. differential pulse voltammetry was used to detect the adriamycin-MMS in a home-made magnetic cell and the reductive peak potential was at -0.40 V. The peak current was linear with adriamycin concentration in the range of 7.25 x 10(-10) similar to 7.25 x 10(-9) mol/L. The detection limit of 3.6 x 10(-10) mol/L was obtained.