摘要
用HPLC法测定苦参碱阴道凝胶中的有关物质,采用ODS色谱柱,以pH=4.0的0.25%(体积分数)磷酸缓冲溶液-三乙胺-乙腈(体积比为96:0.2:4)为流动相,流速为1.0 mL/min,检测波长为210nm,苦参碱的浓度为0.786~1.834μg/mL时与色谱峰面积有良好的线性关系,相关系数为0.9994。有关物质含量测定结果稳定,相对标准偏差为2.33%。
Using ODS column, 0. 25% ( volume fraction) phosphoric acid buffer solution ( pH =4. 0)-tiethylamine - aceto-nitrile (volume ratio being96:0.2:4) as the mobile, the flow rate is 1.0 mL/min and detection wavelength is 210 nm, the relative substance in matrine vagina gel was determined by HPLC. An excellent linear relationship was obtained between the peak area and the concentration of the matcine in the range of 0. 786-1. 834μg/mL. The results kept the stability with RSD of 2. 33% .
出处
《化学分析计量》
CAS
2003年第4期36-37,共2页
Chemical Analysis And Meterage