摘要
利用PCR技术扩增白介素2受体(IL2R)a亚单位的编码序列,同高效表达载体pMYvrf重组,经过复杂的基因操作,获得了5株人IL2Ra亚单位的重组克隆菌株。其中pMYIL2R_(104)表达水平达菌体总蛋白的41%。经免疫印迹分析证明具有特异性IL2R的抗原性,其与配基结合刺激T细胞增殖等生物活性正在研究之中。表达产物有可能用于IL2纯化,制备IL2R的单抗,免疫抑制效应及ATL治疗等研究。
The DNA fragment containing the full-length coding region of IL2Rα subunit was amplified by PCR technique, and recombined with the high expression vectors pMYvrfl, 2,3 by careful design and complex gene manipulation. As a result, five recombinant IL2R clones were obtained, one of which expressed highly IL2R. The expressed protein accounted for 41% of total bacteria protein, and showed the specific IL2R antigenicity after immunoblotting test. Its biological activities are under study at present. The expressed product may be applied to purification of IL2, production of monoclonal antibodies against interleukin 2 receptor and potential treatment for adult T cell leukemia (ATL), and so on.
出处
《军事医学科学院院刊》
CSCD
北大核心
1992年第2期91-96,共6页
Bulletin of the Academy of Military Medical Sciences
基金
国家人事部非教育系统归国留学人员基金
关键词
受体
表达载体
白细胞介素
interleukin 2(IL2)
interleukin 2 receptor(IL2R)
PCR
expression vector
reading frame
immunoblotting
