摘要
目的 :探讨用异硫氰酸苯酯 (PITC)衍生法高效液相色谱检测 3 甲基组氨酸 (3 MH)和酪氨酸 (Tyr)。 方法 :将PITC作为高效液相色谱柱前衍生剂 ,干燥后存放在冰箱 ,样品进样后 ,用乙酸钠和乙腈作为二元梯度洗脱5 0min完成分析。 结果 :本实验中建立的方法 3 MH和Tyr均具有良好的线性 ,相关系数分别为 0 9991与 0 .9992 ,回收率为 82 6 %与 87 6 %。 结论 :PITC衍生法高效液相色谱检测 3 MH和Tyr稳定可靠 ,灵敏度高 。
Objectives:To determine 3-methylhistidine(3-MH) and tyrosine(Tyr) in standard solution and preparations by high performance liquid chromatography(HPLC) with phenylisothicyanate(PITC) pre-column derivation. Methods:PITC was used as pre-column derivative of HPLC and stored in refrigerator after drying.Two phases gradient elution was performed with acetic sodium and cyanide for 50 minites. Results:The linearity of the measurement was very good for 3-MH and Tyr(r= 0.999 1 and 0.999 2).The recovery rate was 82.6% and 87.6%,respectively. Conclusions:The method of HPLC with PITC pre-column derivation for determination of 3-MH and Tyr is stable,efficient,and sensitive.PITC is an ideal derivative.
出处
《肠外与肠内营养》
CAS
2003年第3期181-182,共2页
Parenteral & Enteral Nutrition
