摘要
以 IGFBP- 3基因作为侯选基因 ,对秦川牛群体中 IGFBP- 3位点与秦川牛高档牛肉产量的关系进行了分析。结果发现 ,不同基因型对秦川牛高档牛肉产出率有一定影响 ,AA、AB及 BB型个体的屠宰率、净肉率及西冷、牛柳、眼肉和嫩肩肉的产率逐渐降低 ,但差异不显著 (P>0 .0 5 ) ;AA型个体的眼肌面积大于 BB型个体 (P<0 .0 5 ) ,AB型和 BB型个体胴体脂肪含量高于 AA型个体 (P<0 .0 1)
In this study,DNA samples from 13 Qinchuan cattle (Bos taurus) were analyzed with PCR RFLPs This locus showed three genotypes,namely AA,AB and BB after electrophoresis.Frequencies of the genotype AA,AB,BB and allele A,B were 0.69,0.31,0 and 0.845,0.155,respectively.This polymorphic locus of IGFBP 3 gene was at Hardy Weinberg equilibrium ( P >0.05).The genotypes of AA,AB,BB slightly affected high quality beef cutting of Qinchuan cattle.Striplion percentage,Tenderloin percentage,Ribeye percentage and Tender shoulder percentage were decreased with the genotypes of AA,AB and BB in Qinchuan cattle,but it was not significant ( P >0.05).Average ribeye area in individuals of AA genotype was significantly higher than that in individuals of BB genotype ( P <0.05),and beef fat content in individuals of genotype AB and BB was significantly higher than that in individuals of AA genotype ( P <0.01).
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2003年第4期67-70,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家"8 63"计划项目 ( 2 0 0 1AA2 43 0 5 2 )
国家自然科学基金项目 ( 3 0 0 70 5 5 1)
杨凌示范区专项基金 ( 99KG8)
农业部留学回国人员活动基金 ( 1999-12 D)
西北农林科技大学青年基金资助项目 ( 0 80 8)
关键词
秦川牛
牛肉产量
分子标记
IGFBP-3基因
产出率
净肉率
眼肌面积
脂肪含量
Qinchuan cattle
high quality beef cutting yield
insulin like growth factor binding protein 3 (IGFBP 3) gene
DNA polymorphism