秦川牛高档牛肉产量的分子标记研究

Polymorphisms of insulin-like growth factor binding protein-3 gene and its associations with several carcass traits in Qinchuan Cattle

以 IGFBP- 3基因作为侯选基因 ,对秦川牛群体中 IGFBP- 3位点与秦川牛高档牛肉产量的关系进行了分析。结果发现 ,不同基因型对秦川牛高档牛肉产出率有一定影响 ,AA、AB及 BB型个体的屠宰率、净肉率及西冷、牛柳、眼肉和嫩肩肉的产率逐渐降低 ,但差异不显著 (P>0 .0 5 ) ;AA型个体的眼肌面积大于 BB型个体 (P<0 .0 5 ) ,AB型和 BB型个体胴体脂肪含量高于 AA型个体 (P<0 .0 1)

In this study,DNA samples from 13 Qinchuan cattle (Bos taurus) were analyzed with PCR RFLPs This locus showed three genotypes,namely AA,AB and BB after electrophoresis.Frequencies of the genotype AA,AB,BB and allele A,B were 0.69,0.31,0 and 0.845,0.155,respectively.This polymorphic locus of IGFBP 3 gene was at Hardy Weinberg equilibrium ( P >0.05).The genotypes of AA,AB,BB slightly affected high quality beef cutting of Qinchuan cattle.Striplion percentage,Tenderloin percentage,Ribeye percentage and Tender shoulder percentage were decreased with the genotypes of AA,AB and BB in Qinchuan cattle,but it was not significant ( P >0.05).Average ribeye area in individuals of AA genotype was significantly higher than that in individuals of BB genotype ( P <0.05),and beef fat content in individuals of genotype AB and BB was significantly higher than that in individuals of AA genotype ( P <0.01).