氮源与无机盐对高浓度酒精发酵的影响

Study on the effects of nitrogen sources and inorganic salts on ethanol fermentation

在完全合成培养基条件下 ,就尿素、硫酸铵 2种氮源和硫酸镁、磷酸二氢钾、氯化钙 3种无机盐对 1株产高浓度酒精的酿酒酵母的间歇发酵影响进行了初步研究。结果表明 ,与尿素相比硫酸铵是较好的无机氮源 ,发酵终了酒精浓度可达 119.9g/L;并初步得到该株酵母发酵所需各无机盐的临界浓度分别为 :硫酸镁 5 g/L,磷酸二氢钾 5 g/L(或 <5 g/L) ,氯化钙 5 g/L。

The effects of nitrogen sources and inorganic salt on high ethanol fermentation in synthetic medium were studied in this paper.Ammonium sulfate was the better nitrogen source between urea and ammonium sulfate,the ultimate ethanol concentration was 119.9 g/L when we used ammonium sulfate as nitrogen source.Moreover,the effects of calcium chloride,magnesium sulfate and potassium biphosphate on high ethanol fermentation were analyzed,the critical concentrations of these inorganic salt the Saccharomyces cerevisiae needed were gained.