rhBMP-2对骨骼肌卫星细胞增殖与粘附的影响

Effects of recombinant human bone morphogenetic protein-2 on the proliferation and adhension of skeletal muscle satellite cells

目的:探讨人重组骨形态发生蛋白(rhBMP)-2对骨骼肌卫星细胞增殖与粘附的影响。方法:体外分离与培养骨骼肌卫星细胞,分别用0、50、100、500、1000ng/ml的rhBMP-2诱导培养基培养48h。利用MTT法测定细胞增殖能力的变化,通过荧光法测定接种后1h的粘附细胞率。结果:rhBMP-2可促进骨骼肌卫星细胞的增殖,这种作用从BMP浓度为500ng/ml即可表现出来,并随着浓度的增加而越发明显。在rhBMP-2作用下骨骼肌卫星细胞的粘附率增高,在500ng/ml的浓度时达最高,但当BMP浓度进一步增加时,细胞粘附率却不再增加。结论:rhBMP-2可促进骨骼肌卫星细胞的增殖,增强其粘附特性。

Objective:To investigate the effects of recombinant human bone morphogenetic protein-2on the proliferation and adhension of skeletal muscle satellite cells.Method:Skeletal muscle satellite cells were harvested and cultured in vitro.The0ng/ml,50ng/ml,100ng/ml,500ng/ml,1000ng/ml rhBMP-2were used to induce skeletal muscle satellite cells for48hours.The attached cells were calculated with fluorescence method at1h after seeding.Cell proliferation was measured with MTT method.Result:The number of attached cells have increased after the cells were induced with rhBMP-2when the concentration below500ng/ml,and reached the highest number when500ng/ml BMP.While the number did not increase with the increase of BMP concentration.rhBMP-2promoted cell proliferation when the concentration was above of500ng/ml.Conclusion:rhBMP-2enhances the adhension and proliferation of skeletal muscle satellite cells.Author's address Nanfang Hospital,First Military Medical University,Guangzhou,510515