中国2001年11省(市)食品中李斯特菌污染状况的主动监测

Active surveillance on Listeria monocytogenes in seven kinds of food in 11 provinces of China in 2001

目的 监测中国主要食品中单核细胞增生李斯特菌 (LM )的污染状况。方法 在 11个省 (市 )抽检生肉、熟肉、生奶、生食蔬菜、酸奶、冰淇淋、生食水产品 7类食物样品 4 0 34份 ,采用国家标准方法 (国标法 )检测LM ,并经BAX○R 系统 (DuPontQualicon ,Wilmington ,DE)鉴定。 结果 4 0 34份样品中 ,国标法检出LM 70株 ,分布于 7省 (市 ) 4类食品中 ,总阳性率为 1.74 % ,11省 (市 )中福建省LM阳性率最高 ,7类食品中生肉污染最严重。与国标法相比 ,BAX○R 系统鉴定结果敏感性 >98% ,特异性 >97%。结论 各省 7类食品不同程度受到LM污染 ,各地应加强相应卫生监管工作。BAX○R 系统可用于准确、快速检测LM。

Objective To carry out national active surveillance on Listeria monocytogenes in foods in China. Methods Four thousand and thirty-four random samples from raw meat, meat product, raw milk, vegetable, yoghurt, icecream and aquatic product were collected in 11 provinces (cities), and examined for Listeria monocytogenes according to the national standard method and confirmed by BAX ○R system (DuPont Qualicon, Wilmington, DE). Results Seventy isolates four kinds of foods in seven provinces were found to have LM according to the national standard method with a total isolate rate of 1.74 %. In Fujian, the rate was higher than in the other provinces. Raw meat was found to be most heavily contaminated in seven kinds of foods. Comparing to national standard method, BAX ○R system showed good sensitivity (>98%) and specificity (>97%). Conclusion In each province seven kinds of food were all contaminated by Listeria monocytogenes to some degrees, suggesting that local sanitary surveillance should be strengthened. BAX system can be used to correctly and quickly screen Listeria monocytogenes .