辽宁省艾滋病病毒1流行株膜蛋白V3环氨基酸变异特点

Mutation of envelop protein V3 loop in HIV-1 epidemic in Liaoning province

目的 了解辽宁省艾滋病病毒 1(HIV 1)感染者 艾滋病 (AIDS)患者体内不同亚型HIV 1膜蛋白V3环氨基酸序列特征 ,突变种类特点。方法 提取含有整合HIV 1前病毒的基因组DNA ,巢式聚合酶链反应 (nest PCR)扩增后直接测序 ,并做序列排列比对、翻译和分析。结果 辽宁省HIV 1感染者感染病毒分属A、B’、C、G四种亚型 ,AIDS患者体内病毒V3环发生与T嗜性 SI表型有关的氨基酸突变形式 (11位出现R ,13位出现S、T、N ,19位出现V ,2 0位出现Y ,2 5、2 9位出现N等 )高于无症状感染组 (P <0 .0 5 ) ,此外还发现GQGR、APGQ、RPGA、GLGR、RPGA等少见的V3环顶端四肽组成形式及第 5位H ,34位S、F等罕见突变形式。结论 辽宁省AIDS人群中 ,A、B、C、G亚型HIV 1毒株V3环各位置出现氨基酸突变情况与国外对B亚型毒株的研究结果总体上相似 ,但发现一些罕见突变和少见V3环顶端四肽组成形式。

Objective To study the characteristics of human immunodeficiency virus (HIV-1) V3 loop amino acid mutations among HIV-1 infected people in Liaoning province. Methods The whole blood samples of the HIV carriers and AIDS patients were collected in Liaoning province, China and were extracted PBMC genome DNA. HIV-1 V3 and flanking region sequences were amplified by nest-polymerase chain reaction (nest-PCR) with env specific primers: ED5/ED12 and ED31/ED33. Products were sequenced directly and sequences were aligned, translated and analyzed. Results In AIDS group, some amino acid mutations at specific position of V3 loop: S to R at position 11, H to S,T and N at position 13, A to V at position 19, F to Y at position 20, Q or D to N at position 25 and 29, were found and all common mutations were associated with T tropic/SI phenotype. The frequency of such amino acid mutations in specific positions was higher in AIDS group than that of the asymptomatic infection group ( P < 0.05 ). In addition, we found some unusual tetramer compositions on the tip of V3 loop: GQGR, APGR and RPGA, GLGR, RPGA in addition to some rare mutations, such as: N to H at position 5 and H to S, F at position 34. Conclusion The amino acid mutations on the V3 loop of HIV-1 epidemic in Liaoning province were in agreement with the results of subtype B, but we observed some rare mutations and unusual tetramer compositions on the tip of V3 loop.