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Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721

Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721
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摘要 Objective: To induce changes in biological character of human liver cancer cell line SMMC-7721 by blocking the expression of telomerase genes hTRT and to explore its value in cancer gene therapy. Methods: The vehicle for eukaryotic expression of antisense hTRT was constructed and then transfected into SMMC-7721 cells. The effects of antisense hTRT gene on telomerase activity, cancer cell growth and malignant phenotypes were analyzed. Results: The obtained transfectants that could express antisense hTRT gene stably showed marked decrease in telomerase activity; the shortening of telomere was obvious; cells presented contact growth inhibition; in nude mice transplantation, the rate of tumor induction dramatically decreased. Conclusion: Antisense hTRT gene expression can significantly inhibit telomerase activity of cancer cells and decrease malignant phenotypes in vitro and in vivo. Therefore, as a telomerase inhibitor, antisense hTRT gene may be a new pathway for cancer therapy. Objective: To induce changes in biological character of human liver cancer cell line SMMC-7721 by blocking the expression of telomerase genes hTRT and to explore its value in cancer gene therapy. Methods: The vehicle for eukaryotic expression of antisense hTRT was constructed and then transfected into SMMC-7721 cells. The effects of antisense hTRT gene on telomerase activity, cancer cell growth and malignant phenotypes were analyzed. Results: The obtained transfectants that could express antisense hTRT gene stably showed marked decrease in telomerase activity; the shortening of telomere was obvious; cells presented contact growth inhibition; in nude mice transplantation, the rate of tumor induction dramatically decreased. Conclusion: Antisense hTRT gene expression can significantly inhibit telomerase activity of cancer cells and decrease malignant phenotypes in vitro and in vivo. Therefore, as a telomerase inhibitor, antisense hTRT gene may be a new pathway for cancer therapy.
作者 牟娇 李晓冬 杨庆 贾凤岐 卫立辛 郭亚军 吴孟超
机构地区 Cancer Institute Tumor Immunology and Gene Therapy Center Tumor Immunology and Gene Therapy Center
出处 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第3期170-174,共5页 中国人民解放军军医大学学报（英文版）
基金 Supported by National 973 Project of China(No.G2000057001)
关键词 hTRT antisense gene gene expression inhibition of telomerase 端粒末端转移酶 hTRT 基因表达 肝癌 SMMC-7721细胞系
分类号 R735.7 [医药卫生—肿瘤]
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  • 1[1]Morin GB. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats [J]. Cell, 1989;59(3): 521.
  • 2[2]Nair SK, Heiser A, Boczkowski D et al. Induction of cytotoxic T cells responses and tumor immunity against unrelated tumors using telomerase reverse transcriptase RNA transfected dendritic cells [J]. Nat Med, 2000;6(9): 1011.
  • 3[3]Meyerson M, Counter CM, Eaton E et al. hTEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization [J]. Cell, 1997;90(4):785.
  • 4[5]Broccoli D, Young JW, de Lang T. Telomerase activity in normal and malignant hematopoietic cells [J]. Proc Natl Acad Sci USA,1995;92(20): 9082.
  • 5[6]Hohn WC, Counter CM, Weinberg RA et al. Creation of human tumor cells with defined genetic element [J]. Nature, 1999; 400(6743): 464.
  • 6[7]Avilion AA, Piatyszek MA, Gupta J et al. Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues [J]. Cancer Res, 1996; 56(3): 645.
  • 7[8]Kyo S, Kanaya T, Takakura M et al. Human telomerase reverse transcriptase as a critical determinant of telomerase activity in normal and malignant endometrial tissues [J]. Int J Cancer, 1999; 80(1): 60.
  • 8[9]Hahn WC, Stewart SA, Weinberg RA et al. Inhibition of telomerase limits the growth of human cancer cells [J]. Nat Med,1999;5(10):1164.
  • 9[10]White LK, Wright WE, Shay JW. Telomerase inhibitors [J]. TRENDS Biotechnol, 2001;19(3):114.
Journal of Medical Colleges of PLA(China)
2003年 第3期

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