助孕3号方防治大鼠自然流产的机理研究

Study on Mechanism of Zhuyun No. 3 in Preventing and Treating Spontaneous Abortion

目的:研究助孕3号方防治肾虚、脾虚型大鼠自然流产的机理。方法:(1)以羟基脲与米非司酮(RU486)建立肾虚加黄体抑制模型,利血平与RU486建立脾虚加黄体抑制模型。(2)观察助孕3号方对病证结合模型大鼠孕酮(P)分泌、蜕膜孕激素受体(PR)和雌激素受体(ER)的影响;酶联免疫法检测血清卵泡刺激素、雌二醇和P,免疫组化法检测蜕膜PR,光镜观察子宫、卵巢的形态学改变。(3)助孕3号方对大鼠离体与在体子宫兴奋性的影响,采用水提液与含药血清进行离体子宫兴奋试验。结果:(1)助孕3号方能提高肾虚加黄体抑制与脾虚加黄体抑制模型大鼠蜕膜PR阳性率,与单纯黄体抑制模型组比较差异均有显著性(P<0.05)。(2)助孕3号方水提液和含药血清均能降低离体子宫肌条收缩的强度(P<0.05),在体子宫兴奋试验提示助孕3号组子宫收缩强度显著下降(P<0.05)。结论:补肾和补肾健脾中药保胎的主要作用环节是提高靶细胞PR以及对子宫平滑肌的舒缓作用。

Objective:To study the mechanism of Zhuyun No. 3 (ZY3) in preventing and treating spontaneous abortion. Methods: Two types (Shen-deficiency type and Pi-deficiency type) of corpus luteum inhibitory abortion rat model were established by hydroxyurea and mifepristone respectively for observing the effect of ZY3 on secretion of progesterone (P) and estradiol (E2), and their receptor (PR and ER) in deciduas by ELISA and im-munohistochemical analysis. And the morphological changes of uterus and ovary were observed with light microscopy. Moreover, the water extract of ZY3 was used to observe its effect on uterine excitability in vivo and drug serum of ZY3 was used to test that in vitro. Results: ZY3 could significantly increase the positive rate of PR in deciduas of model of both types (P<0.01), its water extract and drug serum could lower the potency of contractility of uterus in vivo and isolated uterine smooth muscle in vitro respectively (both P<0.01). Conclusion : The major mechanism of TCM drugs for reinforcing Shen and strengthening Pi in protecting pregnancy is to elevate the target cell PR and to relax the uterine smooth muscle.