柠檬酸铁对舟形藻生长及理化成分的影响

Effects of Ferrite on the Growth and Biochemical Compositions of Navicula Lenzi

微藻的生长受很多因素的影响,文章通过对海洋底栖硅藻进行培养,研究培养基中柠檬酸铁浓度对舟形藻生长及理化成分的影响,为舟形藻的扩大培养及在饵料方面的大规模应用提供理论基础。实验方法:静置培养,每天摇瓶3次,考马斯亮蓝法测定蛋白质含量,蒽酮法测定多糖含量,分光光度法测定色素含量,改良的酸水解法测定粗脂含量。实验结果如下:当培养基中的柠檬酸浓度为2 mg·L^(-1),最大细胞密度为4.97×10~4个·mL^(-1),而增长率也最大,达0.076 d^(-1)。藻细胞的理化成分受柠檬酸铁浓度的影响显著,当培养基中柠檬酸铁浓度为8 mg·L^(-1)时,单个藻细胞中蛋白质含量、多糖含量、粗脂含量均为最高,当柠檬酸浓度为4 mg·L^(-1)时,藻类的叶绿素和类胡萝卜素含量最高。由此可见,高浓度柠檬酸铁有利于胞内理化成分的积累,低浓度柠檬酸铁有利于舟形藻细胞的生长。

The growth of microalgae was affected by many factors,culture of benthic diatom,to study the effect of ferric citrate on the growth and biochemical compositions of Navicula Lenzi,it can provide a theoretical basis of the training and large-scale applications.When the ferric citrate was 2 mg?L-1,the highest density of algal cell 4.97×104 mL-1,the highest growth rate 0.076 d-1.Biochemical compositions of algal cells were significantly affected by the different concentrations of ferric citrate,the most protein content,polysaccharide content,crude fat content were obtained under the ferric citrate is 8 mg?L-1,high concentrations of ferric citrate were benefit to accumulation of biochemical compositions,but not conducive to the growth of cells.When the ferric citrate was 4 mg?L-1,the content of chlorophyll and carotenoid was highest.Thus,the increasing ferrite can elevate biochemical compositions of algal cells,the low concentration of ferrite can promote the growth of Navicula lenzi.