白补药酚酸含量及抗氧化活性的测定

Study on Phenolic Acids and Antioxidant Activity of Salvia scapiformis Detected by UV Spectrophotometry

以丹参素钠为对照,采用紫外分光光度法测定雷公山野生白补药酚酸的含量,并通过对羟基自由基(·OH)清除率的测定,评价了白补药酚酸的抗氧化活性.实验结果表明,雷公山野生白补药酚酸的含量为77.25 mg·g-1,该测定方法快速、简便、可靠,可用于白补药酚酸的含量测定.对羟基自由基(·OH)的清除率明显高于阳性对照VC和BHT,EC50分别为2.99μg·m L-1,4.82μg·m L-1和9.12μg·m L-1,说明雷公山野生白补药酚酸抗氧化活性较高,有一定的开发价值.

Tanshinol was as control,phenolic acids of Salvia scapiformis and ·OH clearance were detected,and antioxidant activity of tanshinol of Salvia scapiformis was evaluated. The result showed that the content of phenolic acids of Salvia scapiformis in Leigongshan was 77. 25 mg · g-1. The method was fast,convenient and reliable,can be used to detect phenolic acids of Salvia scapiformis. ·OH clearance of Salvia scapiformis was significantly higher than VC and BHT. EC50 was respectively 2. 99μg·m L-1,4. 82 μg·m L-1and 9. 12 μg·m L-1. The antioxidant activity of phenolic acids of Salvia scapiformis was high,can be developed.