平阳霉素化疗对口腔鳞癌bcl-xl、Bak蛋白表达的影响

Modulation of bcl-xl and Bak protein of oral squamous cell carcinoma treated with pingyangmycin

目的探讨平阳霉素化疗对口腔鳞癌bcl-xl、Bak蛋白表达的影响,进一步了解平阳霉素用于口腔鳞癌化疗的机制。方法从第四军医大学口腔医学院口腔颌面外科及解放军第150中心医院口腔科接受平阳霉素术前诱导化疗的口腔鳞癌病例中筛选出平阳霉素化疗为初始治疗的患者,有效组和无效组均为20例,用免疫组织化学染色方法检测每位患者用药前后组织标本中bcl-xl、Bak蛋白表达水平。结果有效组平阳霉素化疗后的口腔鳞癌标本中bcl-xl蛋白表达明显降低(P<0.01),而Bak蛋白的表达水平无明显改变(P>0.05)。而无效组bcl-xl、Bak蛋白的表达均无明显变化(P>0.05)。结论有效组中平阳霉素能有效地下调口腔鳞癌bcl-xl蛋白的表达。

Objective To assess the alteration of bcl-xl and Bak protein level on oral squamous cell carcinoma treated with pingyangmycin. Methods The total 40 cases of oral squamous cell carcinoma,previously untreated,which had received preoperative chemotherapy of pingyangmycin in author's hospital were divided into two groups: responders(CR+PR:20cases) and nonresponders(SD+PD:20cases). The pro-and after treated specimen were analyzed which were obtained from the operation for 40 cases. The expression level of bcl-xl and Bak protein was detected with immunohistochemical method. Results Pingyangmycin can decrease the level of bcl-xl protein in responders(P <0.01),but not change the level of Bak protein in responders and bcl-xl protein,Bak protein in noresponders(P >0.05). Conclusion Pingyangmycin can downregulate the level of bcl-xl protein in responders.