期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

肠三叶因子在胃黏膜应激性损伤中的修复作用 被引量：1

Role of the ITF in repairing of stress-induced gastric lesions
下载PDF
导出
摘要 目的:研究肠三叶因子(ITF,intestinal trefoil factor,为三叶肽家族一员)在水浸束缚应激(WRS)大鼠胃黏膜基因表达的变化,探讨其在应激胃黏膜损伤中的修复作用.方法:采用单次及单次和重复水浸束缚应激制作模型,动态监测胃黏膜血流量(GMBF),大体及光镜下观察黏膜损伤程度(UI)及组织学变化,逆转录-多聚酶链反应(RT-PGR)检测ITF基因表达变化,免疫组化染色进一步证实ITF表达.结果:单次应激造成胃黏膜广泛损伤,但损伤指数在2、4、8 h逐渐减小,至8 h降为64.9%,GMBF逐渐恢复,至8 h上升为正常的89.8%,ITF基因表达增强(0.022±0.001vs0.177±0.010 P＜.01),免疫组化染色计分为(0.134±0.001 vs0.253±0.01,P＜0.01);单次和重复应激后胃黏膜产生适应性,胃黏膜血流量上升,损伤逐渐减轻,4次应激后,损伤指数降低为单次应激的22.0%,胃黏膜血流量上升为正常的94.2%,并且胃腺区细胞增生,ITF基因表达增强(0.040±0.001 vs0.372±0.01,P＜0 01),免疫组织化学染色计分为(0.134±0.001 vs0.354±0.07,P＜0.01).结论:ITF不仅可能参与胃黏膜早期重建(上皮移行),还有可能参与胃黏膜慢修复反应(细胞增生).
作者 李兆申 聂时南 湛先保 龚燕芳 屠振兴 许国铭
机构地区 中国人民解放军第二军医大学长海医院消化内科
出处 《世界华人消化杂志》 CAS 2003年第7期994-996,共3页 World Chinese Journal of Digestology
基金 军队医药卫生科研项目
关键词 肠三叶因子 胃黏膜应激性损伤 修复 大鼠 基因表达 胃黏膜血流量
分类号 R573 [医药卫生—消化系统] Q516 [生物学—生物化学]
  • 相关文献

参考文献3

二级参考文献13

  • 1林三仁.加强对胃粘膜保护机制的研究[J].胃肠病学,1999,4(3):131-132. 被引量：21
  • 2Cook GA,Thim L,Yeomans ND,et al.Oral human spasmolytic polypeptide protects against aspirin induced gastric injury in rats[].Journal of Gastroenterology.1998
  • 3Kanai M,Mullen C,Podolsky DK.Intestinal trefoil factor induces inactivation of extracellular signal-regulated protein kinase in intestinal epithelial cells[].Proceedings of the National Academy of Sciences of the United States of America.1998
  • 4Konturek PC,Brzozowski T,Pierzchalski P,et al.Activation of genes for spasmolytic peptide, transforming growth factor alpha and for cyclooxygenase(COX)-1 and COX-2 during gastric adaptation to aspirin damage in rats[].Alimentary Pharmacology and Therapeutics.1998
  • 5Liu D,el-Hariry I,Karayiannakis AJ,et al.Phosphorylation of βcatenin and epidermal growth factor receptor by intestinal trefoil factor[].Laboratory Investigation.1997
  • 6Tanaka S,Podolsky DK,Engel E,et al.Human spasmolytic polypeptide decreases proton permeation through gastric mucus in vivo and in vitro[].American Journal of Physiology.1997
  • 7Williams R,Stamp GW,Gilbert C,et al.PS2 transfection of murine adenocarcinoma cell line 410. 4 enhances dispersed growth pattern in a 3-D collagen gel[].Journal of Cell Science.1996
  • 8Tomasetto C,Masson R,Linares JL,et al.pS2/TFF1 interacts directly with the VWFC cysteine-rich domains of mucins[].Gastroenterology.2000
  • 9Ludeking A,Fegert P,Blin N,et al.Osmotic changes and ethanol modify TFF gene expression in gastrointestinal cell lines[].FEBS Letters.1998
  • 10Podolsky DK.Mechanisms of regulatory peptide action in the gastrointestinal tract: trefoil peptides[].Journal of Gastroenterology.2000

共引文献20

同被引文献8

  • 1STUDIER FW, ROSERBERG AH, DUNN JJ, et al. Use of T7 RNA polymerase to direct expression of cloned genes [J]. Methods Enzymol, 1990, 185:60-89.
  • 2DUBERDORFF JW, STUDIER FW. Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter [J]. J Mol Biol, 1991, 219( 1 ):61-68.
  • 3LAVALLIE ER, DIBLASIO EA, KOVACIC S, et all A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm [J]. Biotechn ology, 1993, 11 ( 2 ): 187-193.
  • 4STEWART EJ, ASLUND F, BECKWITH J. Disulfide bond formation in the Escherichia coli cytoplasm: an in vivo role reversal for the thioredoxins [J]. EMBO J, 1998, 17( 19): 5543-5550.
  • 5SUEMORI S, LYNCH-DEVANEY K, PODOLSKY DK. Identification and characterization of rat intestinal trefoil factor: tissue- and cell-specific member of the trefoil protein family [J]. Proc Natl Acad Sci USA, 1991, 88:11017-11021.
  • 6EFSTATHIOU JA, NODA M, ROWAN A, et al. Intestinal trefoil factor controls the expression of the adenonmatous polyposis colicatenin andthe Ecadherin-catenin complexes in human colon carcinoma cells [J]. Proc Natl Acad Sci USA, 1998, 95(6): 3122-3127.
  • 7TAUPIN D, PEDERSEN J, FAM1LARI M, et al. Augmented intestinal trefoil factor (TFF3) and loss ofpS2 (TFF1) expression precedes metaplastic differentiation of gastric epithelium [J]. Lab Invest, 2001, 81:397-408.
  • 8聂时南,李兆申,屠振兴.三叶肽对胃粘膜保护机理的研究进展[J].临床内科杂志,2002,19(2):98-101. 被引量：2

引证文献1

二级引证文献1

世界华人消化杂志
2003年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部