端粒酶逆转录酶mRNA在舌鳞癌和癌前病变中的表达

EXPRESSION OF TERTmRNA IN PRECANCEROUS LESION AND SQUAMOUS CELL CARCINOMA OF TONGUE

目的 :探讨端粒酶活性在正常舌黏膜、上皮单纯性增生、上皮异常增生和舌鳞癌中的表达。方法 :用原位杂交法检测 8例正常舌黏膜 ,8例上皮单纯性增生 ,2 7例上皮异常增生和 5 2例舌鳞癌中端粒酶逆转录酶 (TERT) m RNA的表达。结果 :TERTm RNA 阳性率在正常舌黏膜和上皮良性增生中为 0。在上皮异常增生中为 4 8.1 % (1 3/ 2 7) ;在舌鳞癌中为 84 .6 %(4 4 / 5 2 )。TERTm RNA阳性率与舌癌患者局部淋巴结转移相关 (P =0 .0 4 2 ) ,与 TNM分期和组织学分级无关 (P =0 .0 91和P =0 .6 71 )。TERTm RNA阳性表达患者生存率低于阴性表达患者 (P =0 .0 2 3)。结论 :端粒酶活性可能在舌鳞癌的发生上起了关键作用。TERTm

Objective:To observe the expression of telomerase reserve transcriptase (TERT)mRNA in ton gue precancerous lesion and squamous cell carcinoma(SCC).Methods:95 cases were delected by insitu hybridization technique for TERTmRNA,including 8 cases of normal tongue mucosa,8 cases of hyperplasia lesion,27 cases of precancerous lesion (epithelial dysplasia) and 52 cases of tongue SCC.Results:The detected positive rate of TERTmRNA varied as follows: 0%(0/16) in normal tongue mucosa and hyperplasia lesion,48 1%(13/27) in precancerous lesion and 84 6%(44/52) in tongue SCC.The expression of TERTmRNA was associated with lymph node metastasis (P=0 042). The positive expression of TERTmRNA correlated with lower survival rate (P=0 023).Conclusion:The activity of telomerase may be a crucial step in tongue carcinogenesis and can be helpful to identify biological behavior and predict the prognosis of patients with SCC of tongue.