FQ-RT-PCR法检测脐带血中β与γ珠蛋白mRNA的水平

MEASUREMENT OF β-AND γ-mRNA LEVELS IN THE UMBILICAL CORD BLOOD BY FLUORESCENCE QUANTITATIVE RT-PCR

目的 :探讨新生儿脐带血中 β与 γ珠蛋白 m RNA水平及其意义。方法 :用实时荧光定量逆转录—聚合酶链反应技术检测 2 3例新生儿脐带血和 2 5例正常对照组外周血中 β与 γ珠蛋白 m RNA水平。结果 :脐带血中 γ/ (β+γ) m RNA比值 [(4 4 .0 8±2 2 .4 1 ) % ]明显高于正常对照组 [(1 .5 5± 4 .1 4 ) % ](P <0 .0 0 1 )。脐带血中胎儿血红蛋白 (Hb F)比值 [(5 8.1 1± 7.1 9) % ]也明显高于正常对照组 [(0 .6 3± 0 .32 ) % ](P <0 .0 0 1 )。结论 :脐带血中 γ/ (β+γ) m RNA及 Hb F水平增高 ,可能说明出生时 γ珠蛋白仍未完全关闭 。

Objective:To investigate the β and γ mRNA levels in the umbilical cord blood Methods:We measured the β and γ mRNA levels from red cells of 23 newborn umbilical cord blood and 25 normal controls by the fluorescence quantitative RT PCR Result:The mean γ/(β+γ) ratio in the newborn umbilical cord blood[(44 08±22 41)%] was higher than that of normal controls (P<0 001) The mean HbF ratio[(58 11±7 19)%] was significantly higher than that of normal controls [(0 63±0 32)%] (P<0 001).Conclusion:The mean γ/(β+γ) ratio and HbF rate was significantly higher than that of normal controls.It probably indicates that γ hemoglobin still has partial expression when being born.