摘要
利用计算机软件对兔防御素基因进行分析,消除大肠杆菌不表达或低效表达密码子。同时利用相关软件对基因进行分析,消除可能影响融合基因表达的一些因素,构建兔防御素大肠杆菌高效表达载体。通过初步表达分析,融合蛋白表达量为菌体总蛋白的27.7％。
The rabbit defensin NP1 gene, which will be expressed in E.coli, aws analyzed by software, with a view to eliminating those oodons which are not or lower expressed The coding region of defensin NP1 was cloning into plasmids pGEX - 4T - 1, Named pGEX NP1. and transformed E.coli DHSa in order to study the expression of defensin NP1 gene. The results of SDS -PAGE analysis shows, that the recombinant plasmids could express efficiently in DH5α, with the fusion protein taking up about 27.7% of total bacterial protein.
出处
《医学研究通讯》
2003年第7期6-8,共3页
Bulletin of Medical Research
