单核细胞趋化蛋白-1在反复肺炎患儿血清中的表达水平及其与体液免疫功能的相关性分析

Expression of MCP-1 in Serum of Children with Recurrent Pneumonia and its Correlation with Humoral Immune Function

目的探讨单核细胞趋化蛋白-1(MCP-1)在反复肺炎患儿血清中表达水平及其与体液免疫功能相关性。方法选择2017年12月—2018年12月在黄梅县人民医院呼吸内科住院的反复肺炎患儿(70例)和急性肺炎患儿(72例)分别作为反复肺炎组和急性肺炎组,选择同一时期在我院进行健康体检的健康儿童66例作为对照组,检测3组研究对象血清中MCP-1、IgA、IgG、IgM、补体C3和补体C4的水平,并进行相关性分析,绘制受试者工作特征(ROC)曲线,评估MCP-1对反复肺炎患儿的诊断价值。结果与对照组比较,急性肺炎组和反复肺炎组患儿血清中MCP-1水平均增高,血清中IgA、IgG和补体C4水平均降低;与急性肺炎组比较,反复肺炎组患儿血清中MCP-1水平增高,血清中IgA、IgG和补体C4水平均降低;血清中MCP-1水平与IgA、IgG和补体C4水平呈负相关;MCP-1诊断反复肺炎的ROC曲线下面积为0.842(95%CI:0.724~0.943)。以MCP-1水平为117.65ng/ml作为诊断截点,MCP-1诊断反复肺炎患儿的灵敏度和特异度分别为83.93%和72.61%。结论反复肺炎患儿血清中MCP-1水平升高,体液免疫功能降低,MCP-1与体液免疫功能呈负相关,血清MCP-1对反复肺炎患儿具有一定的诊断价值。

Objective To investigate the expression of MCP-1 in serum of children with recurrent pneumonia and its correlation with humoral immune function.Methods From December 2017 to December 2018,70 children with recurrent pneumonia and 72 children with acute pneumonia were selected as the recurrent pneumonia and acute pneumonia groups respectively.66 healthy children who underwent physical examination in our hospital at the same time were selected as the control group.The serum levels of MCP-1,IgA,IgG,IgM,complement C3 and complement C4 were detected and their relationship were investigated.The ROC curve was drawn to evaluate the diagnostic value of MCP-1 in children with recurrent pneumonia.Results Compared with the control group,the serum levels of MCP-1 increased and IgA,IgG and complement C4 decreased in both acute pneumonia and recurrent pneumonia groups.Compared with acute pneumonia group,the serum levels of MCP-1 increased and serum levels of IgA,IgG and complement C4 decreased in recurrent pneumonia group.The serum levels of MCP-1 were negatively correlated with those of IgA,IgG and complement C4.The area under ROC curve of inflammation was 0.842(95%CI:0.724-0.943).The sensitivity and specificity of MCP-1 in children with recurrent pneumonia were 83.93%and 72.61%,respectively when the level of MCP was 117.65 ng/ml.Conclusion Serum MCP-1 level in children with recurrent pneumonia increased and humoral immune function decreased.MCP-1 is negatively correlated with humoral immune function.Serum MCP-1 has certain diagnostic value for children with recurrent pneumonia.