老年散发性大肠腺癌患者GSTT1基因多态性及其临床特征分析

Relationship between genetic polymorphisms of glutathione-S-transferase T1 and the clinico-pathological features of sporadic colorectal adenocarcinoma in the elderly

目的 探讨谷胱甘肽 S 转移酶T1(GSTT1)基因多态性与老年人散发性大肠腺癌易感性及其临床特征。 方法 应用多重聚合酶链反应技术检测GSTT1基因多态性。 结果 GSTT1空白基因型频率在老年散发性大肠腺癌患者 (6 6 7% )、老年远端散发性大肠腺癌患者 (75 5 % )与老年对照组 (5 1 8% )之间的差异均有显著性 (前者 χ2 =4 2 7,P <0 0 5 ;后者 χ2 =7 93,P <0 0 1) ;老年中度分化性大肠腺癌患者 (80 7% )GSTT1空白基因型的频率明显高于老年对照组 (χ2 =8 30 ,P <0 0 1) ;不同临床分期的老年大肠腺癌患者与老年对照组比较 ,GSTT1空白基因型的频率差异均无显著性。 结论 GSTT1空白基因型与老年人散发性大肠腺癌的易感性有关 ,肿瘤多位于大肠远端 ,呈中度分化性腺癌。

Objective To analyze the association of genetic polymorphisms of glutathione-S-transferase T1 (GSTT1) gene with clinico-pathological features of sporadic colorectal adenocarcinoma (CRAC) in the elderly. Methods Multiplex PCR was used in the study of genetic polymorphisms of GSTT1 gene. Results The frequence of null genotype for GSTT1 was higher among sporadic CRAC patients(66.7%) when compared with that of the elderly controls(51.8%) with statistically significant difference (χ 2 =4.27, P<0.05). The null genotype for GSTT1 was more frequent in distal CRAC(75.5%, χ 2 =7.93), and was more frequent in moderately differentiated CRAC(80.7%, χ 2 =8.30) than that of the elderly controls, all showing statistical differences(P<0.01). However, the frequency of GSTT1 null genotype in various tumor stages of the elderly CRAC showed no significant difference compared with the controls. Conclusions The null genotype for GSTT1 is significantly related to the genetic susceptibility of elderly sporadic CRAC, especially to that of the elderly distal sporadic CRAC and moderately differentiated CRAC.