针刺对大鼠局灶性脑缺血再灌注损伤的保护作用

Protective Effect of Acupuncture on Cerebral Cells in Focal Cerebral Ischemia-reperfusion Injury Rats

目的 :观察针刺对大鼠大脑中动脉栓塞再灌注后的神经保护作用。方法 :将 3 0只大鼠随机分为正常组、模型组和针刺组。模型组和针刺组均用栓线插入大鼠大脑中动脉根部 ,2hr后抽出 ,使缺血组织再灌注 ,复制可逆性大脑中动脉栓塞 (rMCAo)模型 ,正常组和模型组不经任何治疗 ,针刺组分别在模型复制前 1hr、模型复制后 8hr和 1 6hr电针“水沟”、“太冲”、“曲池”、“足三里”、“丰隆”。观察神经行为症状 ,2 4hr后断颈处死大鼠 ,检测血液流变学指标 ,脑组织TTC染色后测量脑梗死及水肿体积 ,行病理组织学检查。结果 :神经行为学针刺组与模型组比较差异有显著性 (P <0 0 1 ) ;脑梗死和脑水肿体积百分率针刺组明显低于模型组 (P <0 0 1 ) ;血液流变学中全血比粘度针刺组与模型组比较差异有显著性 (P <0 0 5) ,但血浆比粘度、红细胞压积无明显变化 (P>0 0 5) ;病理组织学观察表明 ,针刺对大鼠脑组织损伤有明显的保护作用。结论

Objective: To study the protective effect of acupuncture on cerebral neurons in cerebral ischenmia-reperfusion injury rats. Methods: Thirty Wistar rats were randomized into control group (n=10), model group (n=10) and acupuncture group (n=10). Cerebral ischemia-reperfusion model was established by reversible occlusion of the middle cerebral artery. Rats of acupuncture group were treated with electroacupuncture of 'Zhigou' (TE 6), 'Taichong' (LR 3), 'Quchi' (LI 11), 'Zusanli' (ST 36) and 'Fenglong' (ST 40) (parameters: 3 Hz, continuous pulses, duration of 15 min, and strength of inducing light tremor of the local muscles), 1 hours before cerebral ischemia, 8 hours and 16 hours after cerebral ischemia respectively. The alterations of neurological symptoms and signs were observed. 24 hours after cerebral ischemia-reperfusion, these rats were killed for collecting blood samples from the tail vein and for taking the brain tissue samples. The indexes of whole blood viscosity, plasma viscosity and hematocrit were detected. The brain tissue was cut into sections to be stained with tetrazolium chloride and HE method separately. Results: Scores of neurological symptoms and signs of model group decreased significantly 24 hours after cerebral ischemia-reperfusion in comparison with those of normal control group and acupuncture group (P<0.01), suggesting an improvement of neurological behavior after acupuncture treatment. In comparison with model group, the volume values of cerebral infarction and brain edema of acupuncture group decreased significantly (P<0.01), and the whole blood relative viscosity was also reduced obviously (P<0.01), but no significant differences were found among the 3 groups in plasma viscosity and hematocrit (P>0.05). Under light microscope, the pathohistological section showed that the neuronal injury of acupuncture group is relatively lighter compared with that of model group. Conclusion: Acupuncture has a protective effect on ischemia-reperfuse damage of cerebral tissue in rats.