艾灸对荷瘤小鼠免疫功能的影响

Influence of Moxibustion on the Immunologic Function of the Tumor-bearing Mice

目的 :观察艾灸对荷瘤小鼠免疫功能的影响。方法 :将 40只荷瘤小鼠随机分为四组 ,对照组、艾灸组、5 氟脲嘧啶 ( 5FU)组、5FU +艾灸组。艾灸组、5FU +艾灸组分别在“中脘”、“神阙”、“关元”穴隔药饼灸 ,隔天 1次 ,治疗 8次。 5FU组、5FU +艾灸组在腹部注射 0 2 5mg/ 0 1mL5FU ,3天 1次 ,共治疗 4次。所有治疗完毕后 2 4hr处死动物。测定小鼠T淋巴细胞、自然杀伤细胞 (NK)及进行淋巴因子激活的杀伤细胞 (LAK)杀伤功能检测。结果 :艾灸组CD3、CD4、CD4/CD8比率、NK细胞、LAK杀伤活性比 5FU组显著提高 (P <0 0 5、P <0 0 0 5、P <0 1、P <0 1、P <0 0 1 ) ,差异明显 ,具有显著性意义。结论 :艾灸能提高小鼠的免疫力 ,而 5FU有抑制作用 。

Objective: To observe the influence of moxibustion on the immunological function of the tumor-bearing mice. Methods: 40 tumor-bearing mice were randomly and evenly divided into control group, moxibustion group, 5Fu (5-fluorouracil) group, 5Fu plus moxibustion group. Herbal cake-separated moxibustion was applied to'Guanyuan' (CV 4),'Shenque'(CV 8) and 'Zhongwan' (CV 12)(herbal cake about 0.5 thick),3 cones each time, once every other day for eight treatments. 0.25 mg/0.1 mL of 5Fu was injected intraperitoneally for 5Fu group and 5Fu plus moxibustion group, once every 3 days, four times in succession. At the end of the treatment as well as 24 hr after completion of treatment, the mice were killed by extraction of the eyeball for collection of blood samples. The tumor tissue was taken for weighting the tumor body. The spleen was taken to be processed into cell suspension for examining the function of T lymphocytes with direct immunofluorescence marking method and flow cytometer. Natural killer cell (NK) activity and lymphokine-activated killer cell(LAK) activity (lethal rates) were detected with an enzyme-linked immunosorbent assay reader for recording OD values. Results: CD3% and CD4%, NK cell and LAK activity of 5FU group were lower than those of control group, suggesting decline of the immunity after injection of 5FU. Comparison between 5FU and moxibustion groups showed that CD3%, CD4% and CD4/CD8 of moxibustion group were significantly higher (P<0.05～0.005) or higher (CD4/CD8) than 5FU group. CD4% of moxibustion plus 5FU group was also significantly higher than that of 5FU group (P<0.005). NK cell activity of moxibustion group, NK cell and LAK cell activity of 5FU plus moxibustion group were all significantly higher than those of 5FU group (P<0.05～0.01), showing an enhancement of the activity of NK and LAK cells by moxibustion. Conclusion: Moxibusion can improve the immune function in tumor-bearing mice.