糖肾康合剂对实验性大鼠糖尿病肾病的预防作用

Preventive Effect of Tangshenkang Mixture on Experimental Diabetic Nephropathy

目的探讨中药复方糖肾康预防实验性大鼠糖尿病肾病的作用。方法链脲佐菌素(STZ)单次腹腔注射制作SD大鼠糖尿病模型。全部大鼠测血糖、尿糖及尿蛋白,并取一侧肾脏切片作HE染色及PAS染色,光镜观察,并在图像分析仪上测量肾小球面积、毛细血管丛面积及PAS染色阳性物质面积。结果①模型组大鼠出现明显蛋白尿,肾小球、毛细血管丛增大,PAS阳性物质增多。②糖肾康组的尿蛋白明显低于模型对照组和西药对照组(P<0.01)。③与模型组比较,糖肾康组肾小球及毛细血管丛增大明显(P<0.01,P<0.05),PAS阳性物质减少(P<0.05)。④两组间血糖、尿糖水平没有显著性差异。结论糖肾康合剂可减轻STZ糖尿病大鼠尿蛋白、减少PAS阳性物质的沉积及阻止肾小球的肥大,无降糖作用。

Objective To evaluate the effect of Tangshenkang Mixture (TM) on preventing diabetic nephropathy (DN). Methods SD rat model of diabetes was established by intraperitoneal injection of streptozotocin (STZ). Contents of blood sugar,urine sugar and urine albumin in rats were measured. The pathological changes of unilateral kidney were also observed under microscope by HE and PAS staining. Glomerulus dimensions,capillary vessel dimensions and PAS-positive matter area were measured with Pathological Picture Analyzing System. Results ①Obvious albuminuria,enlargement of glomerulus and capillary vessel,increase of PAS-positive matter area were found in model group. ②Urinary protein content in TM group was significantly lower than that in model group and western medicine group(P < 0.01).③Compared with model group,the enlargement of glomerulus and capillary vessel in TM group was obvious (P < 0.01,P < 0.05),and the difference of decrease of PAS-positive matter was also significant(P < 0.05). ④The difference of blood sugar and urine sugar between model group and TM group was insignificant. Conclusion TM can relieve albuminuria,decrease the accumulation of PAS-positive matter and prevent glomerulus from enlarging in STZ-iduced diabetic rats but has no effect in reducing blood sugar content.