肺癌患者化疗前后外周血T淋巴细胞亚群及NK细胞的变化

Variation of blood T lymphocyte subsets and NK cells before and after chemotherapy of the lung cancer in patients with peripheral

目的探讨肺癌患者在化疗前后外周血T淋巴细胞亚群和NK细胞的变化。方法采用流式细胞术(FCM)检测80例肺癌患者在化疗前后外周血T淋巴细胞亚群、和NK细胞的百分率,并与80例健康对照组比较并进行统计学分析。结果与健康对照组比较,肺癌组外周血CD3^+、CD4^+、CD4^+/CD8^+、NK(CD16+56+)明显低于健康对照组,CD8^+明显高于健康对照组(P<0.05)。肺癌组化疗后与化疗前比较,CD3^+、CD4^+、CD4^+/CD8^+、NK(CD16+56+)升高,CD8+降低(P<0.05)。结论检测肺癌患者外周血T淋巴细胞亚群和NK细胞的变化,可以了解肺癌患者的机体免疫功能状况,同时可以作为肺癌患者疾病转归、预后判断及临床治疗效果的参考指标之一。

OBJECTIVE To explore the variation of blood T lymphocyte subsets and NK cells before and after chemotherapy of the lung cancer in patients with peripheral METHODS Using flow cytometry(FCM)in 80 cases of lung cancer patients before and after chemotherapy peripheral blood T lymphocyte subsets and the percentage of NK cells,and compared with 80 cases of healthy controls and statistical analysis.RESULTS Compared with healthy controls,lung group of peripheral blood CD3^+,CD4^+,CD4^+/CD8^+,NK(CD16+56+)significantly lower than the healthy controls,CD8^+ are significantly higher than healthy controls(P<0.05).Lung cancer after chemotherapy group compared with before chemotherapy,CD3^+,CD4^+,CD4^+/CD8^+,NK(CD16+ 56+),CD8^+lower(P<0.05).CONCLUSIONS The detection of lung cancer patients with peripheral blood T lymphocyte subsets and NK cells,can understand the status of the immune function in patients with lung cancer,and can be used as the outcome prognosis and judgment of patients with lung cancer disease also can be the reference index of clinical therapeutic effect.