Fas相关死亡域蛋白表达与增生性瘢痕演变的关系(英文)

Relationship between experession of Fas protein associated with death domain with development of hyperplastic scars

目的探讨Fas相关死亡域蛋白(FADD)在增生性瘢痕(HS)各个时期的表达变化以及与HS演变的关系。方法采用原位杂交与图象分析系统相结合的方法,定量观察FADD(2份,取自同一部位样本)在HS增殖期、成熟期和消退期的表达分布及表达量。结果FADD在HS成熟期的表达量犤面度(9.36±0.92),平均光密度(1154±68)〗是增殖期犤面度(4.72±0.35),平均光密度(552±32)〗的2倍,是消退期犤面度(1.88±0.12),平均光密度(218±16)〗的5倍;FADD阳性信号主要见于表皮棘层、真皮网状层,以及部分血管。结论FADD表达与增生性瘢痕演变密切相关。

Aim To explore the change of in situ expression of FADD (Fas protein associated with death domain) at various stages in hypertrophic scars and its implication.Methods The change of expression of FADD genes at mature stage, proliferative stage and regressive stages in HS was quantitatively investigated by in situ hybridization techniques and imagine analysis system.Results Expressive intention of FADD genes at the mature stageRelationship between experession of Fas protein associated with death domain with development of hyperplastic scars$$$$ Bin Shu,Lin-Lin Hao, Zhi-Rong Nie,Yong-Hui Lin,Guang-Rui Feng,Zong-Yao Wu<P>Bin Shu,Lin Lin Hao,Zhi Rong Nie,Yong Hui Lin,Guang Rui Feng,Department of Rehabilitation,Daping Hospital,Third Military Medical University of PLA,Chongqing 400042,China<P>Zong Yao Wu,Department of Rehabilitation,Western South Hospital,the Third Military Medical University of PLA,Chongqing 400038,ChinaABSTRACT:Aim To explore the change of in situ expression of FADD (Fas protein associated with death domain) at various stages in hypertrophic scars and its implication.Methods The change of expression of FADD genes at mature stage, proliferative stage and regressive stages in HS was quantitatively investigated by in situ hybridization techniques and imagine analysis system.Results Expressive intention of FADD genes at the mature stage[area density( 9.36± 0.92) , average integral optical density( 1154?à 68) ?1/2 was 2 times as that at the proliferative stage[area density( 4.72± 0.35) , average integral optical density( 552?à 32) ?1/2 ,and 5 times as that at regressive stage[area density( 1.88± 0.12) , average integral optical density( 218?à 16) ?1/2 .Staining sites located in the stratum spinosum of epidermis,reticular dermis and part vessels.Conclusion FADD expression is tightly associated with the development of hypertrophic scars.<P>