免疫细胞化学技术检测不孕妇女血清抗精子抗体

The detection of serum antisperm antibody of infertile women by immunochemical assay

目的 :分析不孕妇女血清中抗精子抗体与精子的特异结合。方法 :筛选不明原因不孕妇女病例 ,用ELISA检测不孕妇女血清中的抗精子抗体 ,再用免疫细胞化学方法验证抗血清是否与正常人精子特异结合。结果 :ELISA检测 1 0 1例不明原因不孕症妇女血清 ,6例 (5.9% )血清抗精子抗体阳性 ,阳性血清经免疫细胞化学试验显示 ,能与精子特异性结合。结论 :抗精子抗体阳性血清能与精子特异性结合。

Objective:To analyze the specific sperm-binding of serum antisperm antibody o f infertile women. Methods The serum antisperm antibody of unexplained infertil e women was detected by ELISA. Then immunochemical assay was used to detect whet her the antibody in the positive sera bound sperm specially. Results:5.9% of the serum samples from 101 unexplained infertile women was positive in antisperm a n tibody by ELISA. The positive sera were found to bind specially to sperm to some extend by immunochemical assay. Conclusion:The antisperm antibody in the ant isperm-positive sera could bind specially sperm.