摘要
以双低优质油菜品种中双 7号的无菌苗为材料 ,探索了以子叶、下胚轴和幼根为外植体 ,及不同生长调节剂组合对愈伤诱导的影响作用。结果表明 ,生长调节剂是愈伤诱导成功的关键因子 ,2 ,4-D是诱导愈伤所必需的 ,以MS培养基含 1mg·L- 1 2 ,4-D与 2mg·L- 1 6 -BA配比为佳 ;同时子叶优于下胚轴和幼根作为外植体进行愈伤诱导 ,可获得出愈率高、增殖快。
The effects of different growth regulator combinations and explants (cotyledon, hypocotyl and young root) upon callus induction of rape were studied, based on aseptically seedlings of 'Zhongshuang No. 7', a double low elite variety The results indicated that growth regulator was crucial to induce callus of rape, in especial 2,4-D, the optimal induction medium which could induce callus was MS medium supplemented with 1 mg/L 2,4-D and 2 mg/L 6-BA, and as explants, cotyledon was fit for callus induction than hypocotyl and young root, to induce callus with high induction rate, quick growth and good quality
出处
《湖北农业科学》
北大核心
2003年第4期41-43,共3页
Hubei Agricultural Sciences
基金
湖北农学院科研基金重点项目资助(HNZD2 0 0 0 0 1)
