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蛋白质定量方法的合理应用 被引量:4

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摘要 蛋白质定量检测的生物化学方法是现代定量测定蛋白质领域中最为常用的方法。本文对目前常用的蛋白质定量方法的局限性,包括各自的检测限、灵敏度、易受的影响因素和常见的干扰物,以及如何正确选择合适的蛋白质定量方法和标准品作一综述。
出处 《中华国际医药杂志》 2003年第2期126-128,共3页
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  • 1曾群力,翁瑜,陈光弟,鲁德强,姜槐,许正平.1800MHz射频电磁场对人乳腺癌细胞蛋白质表达的影响[J].中华预防医学杂志,2006,40(3):153-158. 被引量:5
  • 2Toyama H,Nishibayashi E,Saeki M,et al.Factors required for the catalytic reaction of PqqC/D which produces pyrroloquinone[J].Biochem Biophys Res Commun,2007; 354(1):290.
  • 3徐叔云,卞廉如,陈修.药理实验方法学[M].2版.北京:人民卫生出版社,1994:781.
  • 4BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding [ J ]. Analytical Biochenmistry, 1976 (72) : 248-254.
  • 5WIECHELMAN K J, BRAUM R D, FITZQATRICK J D. Investigation of the bicinchoninic acid protein assay:identification of the groups responsible for color formation [J]. Anal Biochem, 1988,175 ( 1 ) : 231-237.
  • 6JIN B F, HE K, WANG H X, et al. Proteomics analysis reveals insight into the mechanism of HRas-Mediate transformation [J]. J Proteome Res, 2006,5 (10) : 2815-2823.
  • 7SHEN J,WU M,YU Y. Proteomic profiling of cellular responses to different concentrations of N-Methyl-N'-nitro-N-nitrosoguanidine [ J ]. J Proteome Res, 2006,5 (2) : 385-395
  • 8CHRISTINE V. SAPAN, ROGER L. LUNDBLAD,NICHOLAS C. Price. Colorimetric protein assay techniques [J]. Bioteehnol Appl Biochem, 1999,29,99-108.
  • 9Funakoshi Y, Ichiki T, Shimokawa H, et al. Rho-kinase mediated angiotensin II induced monocyte chemoattractant protein-lexpression in rat vascular smooth musele cells[J]. Hypertension,2001,38(1) :100-104.
  • 10Sauzeau V, Le Mellionnec E, Bertoglio J, et al. Human Urotensin ll-lnduced Contraction and Arterial Smooth Muscle Cell Proliferation Are Mediated by RhoA and Rho-Kinase[J]. Circ Res,2001,88(11):1102-1104.

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