摘要
考察了 Mg2 + 、Ca2 + 、NH+4、PO3-4等无机离子、初始 p H、装液量以及诱导前添加葡萄糖对重组大肠杆菌表达rh ANG和菌体生长的影响。经过优化 ,rh ANG表达量提高约 39% ,菌体干重达 1.5 9g/ L ,为工程菌发酵的扩试提供了参考。
The fermentation process of recombinant human angiogenin was studied. The effects of factors such as concentration of inorganic ions, i.e. Mg 2+ ,Ca 2+ ,NH +__4 and PO 3- __4, pH value, volume of medium and the amount of added glucose before induction on rhANG expression were investigated. The results showed that the expression level of rhANG was increased to about 39% of the total bacteria protein and the biomass reached 1.59 g/L after optimization.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2003年第8期380-382,共3页
Chinese Journal of Pharmaceuticals
关键词
人血管生长素
重组大肠杆菌
发酵条件
recombinant human angiogenin
Escherichia coli
fermentation