腺嘌呤用固定化酵母生产三磷腺苷的初步研究

ATP Formation from Adenine by Immobilized Yeast

采用固定化酵母由腺嘌呤产生三磷腺苷 (ATP)。在含腺嘌呤 2 .2 g/ L、葡萄糖 6 0 g/ L、氯化镁 6 .4 g/ L的 p H7.0、2 0 0 m mol/ L磷酸缓冲液中 ,加入固定化酵母 10 0 0 g/ L (反应液体积 ) ,33°C、转化反应 2 h可生成 2 .32 g/ L的ATP,比游离酵母转化反应时间缩短一半 ,ATP生成率提高 6 8%左右。

The method of producing ATP from adenine by immobilized yeast was presented. Substrate solution contained adenine 2.2 g/L, glucose 60 g/L, MgCl 6.4 g/L in 200 mmol/L potassium phosphate buffer solution adding immobilized yeast 1000 g/L. ATP(2.32 g/L) could be formed at pH 7.0 and 33°C for 2 h. The reaction time by using immobilized yeast was reduced 50% and the ATP formation was relatively increased 68% than that of free yeast.