云南28个群体中含黄素单氧化酶3基因突变频率分布

The frequency distribution of flavin-containing monooxygenase 3 mutant alleles in 28 popul ations from Yunnan

目的 研究含黄素单氧化酶 3(flavin- containing monooxygenase3,FMO3)基因突变频率在云南少数民族群体中的分布。方法 采用聚合酶链反应 -限制性片段长度多态性方法对云南 2 4个少数民族的 2 8个群体进行了 FMO3基因型分析。结果 FMO3/ Stop1 48频率分布范围为 0 .174～ 0 .80 3,平均为0 .395 ;FMO3/ L ys1 58频率分布范围为 0 .0 5 6～ 0 .4 14 ,平均为 0 .2 0 8;FMO3/ Gly30 8频率分布范围为 0～0 .2 17,其中布朗族、新平花腰傣、德宏水傣、壮族、德昂族、景颇族、怒族和回族的频率为零 ,平均为 0 .0 4 6。结论 FMO3基因突变频率不仅民族间有较大的差异 ,而且同一民族不同群体也不相同。

Objective: To study the frequency distribution of flavin-containing monooxygenase 3 (FMO3) mutant alleles in 28 populations originating from 24 ethnic minorities in Yunnan of China. Methods: FMO3 genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results: The average frequencies of FMO3/Stop148, FMO3/Lys158 and FMO3/Gly308 were 0. 395 (0. 174-0. 803), 0. 208 (0. 056-0. 414), 0. 046 (0-0. 217), respectively. The frequencies of FMO3/ Gly308 in Blang, Huayaodai, Shuidai, Zhuang, De'ang, Jingpo, Nu and Hui populations were null. Conclusion: It was found that the frequencies of FMO3 mutant alleles varied not only in different ethnic groups, but also in different populations that stemmed from the same ethnic group.