钙粘附蛋白在人类早期胚胎的表达

Expression of E-cadherin in human embryo

目的 检测钙粘附蛋白在人类早期胚胎的表达 ,探讨钙粘附蛋白在胚胎着床及发育中的作用。方法 应用特异性单克隆抗体 ,采用间接免疫荧光方法 ,检测人种植前胚胎 (分别为 2、4、6、7、8细胞胚及囊胚 )上皮型钙粘附蛋白的表达 ,并应用激光共聚焦显微镜对荧光进行定量检测。结果 各期人种植前胚胎表面均见上皮型钙粘附蛋白表达 ,囊胚期表达明显增高。结论 人类早期胚胎在发育过程中能表达上皮型钙粘附蛋白 ,且表达量逐渐增加 ,可能对胚胎成功着床有重要意义。

Objective: To investigate the role of calcium dependent adhesion molecules (cadherin) during the implantation and development of human embryo by studying the expression of E-cadherin in human embryo and blastocysts. Methods: Expression of E-cadherin in preimplantation embryos and blastocysts was detected by indirect immunofluorescence assay and quantitated by laser scanning confocal microscopy. Results: Expression of E-cadherin was found in all the preimplantation embryos and blastocysts the present authors studied. The expression was higher in blastocysts than that in preimplantation embryos. Conclusion: The above results suggested that human embryos and blastocysts could express E-cadherin and the expression increased during their development. These may be of significance to the adhesion and implantation of the human embryo and blastocysts.