米非司酮抑制人脑膜瘤生长作用机制的体内实验研究

THE MECHANISMS OF MIFEPRISTONE′S ANTITUMOR EFFECTS ON HUMAN MENINGIOMA CELLS IN VIVO

目的 探索米非司酮抑制人脑膜瘤生长的作用机制。方法 应用裸鼠肾包膜下法建立人脑膜瘤动物模型。采用实验剂量的米非司酮皮下用药 ,而对照组只注射溶剂 ,2 8d后将肿瘤生长率作统计学分析。原始标本用免疫组化SP法检测孕激素受体 (PR) ;增殖细胞核抗原 (PCNA)免疫组化法检测细胞增殖情况 ;电镜观察用药前后的肿瘤细胞超微结构改变。结果 实验组与对照组之间有显著性差异 (P <0 0 1) ,免疫组化、PCNA免疫组化以及电镜的结果表明米非司酮可明显抑制脑膜瘤细胞的增殖。结论 米非司酮可能是通过阻断PR/糖皮质激素受体 (GR)

Objective: To explore the mechanisms of mifepristone′s antitumor effects on human meningioma through morphology changes. Methods: Meningioma tissues from 7 patients were implanted into the subrenal capsules of nude mice. The effective doses of mifepristone were given to experiment group for 28 days, and the second group was given sesame oil alone as control. All meningioma tissues were examined for the PR by the immunohistochemitry method; meningioma tissues, including original tissue and the one after mifepristone treatment, were examined with PCNA immunohistochemitry methods. To investigate the changes of histology, electro-microscope examination was applied. Results: Antiprogesterone treatment results in a marked reduction of the tumor volume in experiment group. PR positive was found in four meningioma tissues of 7 patients. PCNA and electromicroscope results suggested that mifepristone could inhibit the reproduction of meningioma cells. Conclusion: This study suggests that mifepristone has an antitumor effect against meningioma cells via the PR and/or other receptor, which perhaps mediates the glucocorticoid receptor.