GPR43在结直肠癌中的表达及其临床意义

Expression of GPR43 in colorectal cancer and its clinical significance

目的分析GPR43在结直肠癌(colorectal cancer,CRC)中的表达情况,以探究GPR43对结直肠癌的发生发展、迁移的可能作用机制。方法留取2016年—2018年于本院肛肠科和病理科诊断为结直肠癌并行结直肠癌根治术的手术标本119例,其对应的癌旁组织119例。采用免疫组化方法(Immunohistochemistry,IHC)在蛋白水平检测结直肠癌组织中GPR43的表达情况,并结合患者的性别、年龄、部位、组织分型、病理分型、分化程度、肿瘤直径、T分期、血管浸润、神经浸润、淋巴结转移等病理相关因素进行统计学分析。结果 GPR43于结直肠癌旁的正常组织中呈现高表达,于细胞膜表面高表达明显,于结直肠癌组织中低表达,且二者表达差异具有统计学意义(P<0.05)。GPR43的表达情况在结直肠癌的研究中具有意义,其在结直肠癌组织中的低表达与结直肠癌的T分期存在显著相关性(P<0.05),差异有统计学意义;与结直肠癌患者的性别、年龄、部位、组织分型、病理分型、分化程度、肿瘤直径、血管浸润、神经浸润、淋巴结转移则无显著相关性(P>0.05)。结论 GPR43在结直肠癌组织中呈低表达及其与T分期的相关性,有助于对结直肠癌的确定诊断,治疗方式的选择进行综合性判断,为结直肠癌的诊断及治疗提供新的理论支持。

Objective To detect expression of GPR43 in Colorectal Cancer( CRC),and to explore the possible mechanism of GPR43 in the occurrence and development,migration of colorectal cancer. Provide a new direction for the diagnosis and treatment of colorectal cancer. Methods From 2016 to 2018,the sample of 119 cases of patients with colorectal cancer diagnosed in the department of anorectal and pathology and received colorectal cancer radical surgery in the first affiliated hospital of Jiamusi University were collected,and 119 cases of adjacent tissues were included. Immunohistochemistry( IHC) was used to detect the expression of GPR43 in colorectal cancer tissues at the protein level. The relative pathological factors including gender,age,location,tissue typing,pathological type,degree of differentiation,tumor diameter,T-stage,blood vessel infiltration,nerve infiltration,and lymph node metastasis were statistically analyzed.Results GPR43 was highly expressed in normal tissues adjacent to colorectal cancer,and was highly expressed on the surface of cell membrane. It was lowly expressed in colorectal cancer tissues,and the difference was statistically significant( P < 0. 05). The expression of GPR43 is of significance in the study of colorectal cancer,and its low expression in colorectal cancer tissues is significantly correlated with the T stage of colorectal cancer( P < 0. 05). The difference is statistically significant. There was no significant correlation between gender,age,location,tissue classification,pathological type,degree of differentiation,tumor diameter,vascular invasion,nerve infiltration,and lymph node metastasis( P>0.05).Conclusions The low expression of GPR43 in colorectal cancer tissues and its correlation with T staging is conducive to the comprehensive judgment of the diagnosis and treatment choice of colorectal cancer,providing new theoretical support for the diagnosis and treatment of colorectal cancer.