五种蓝舌病毒的分子系统(英文)

MOLECULAR PHYLOGENCY OF FIVE BLUETONGUE VIRUSES

对蓝舌病毒(BTV)血清型2、11和13的双链RNA中L3基因的全序列进行了测定,该基因编码这种病毒的重要内壳蛋白质(VP3),应用前人测定的血清型10和17的BTV的L3基因序列,共5种美国存在的BTV的L3基因。该病毒的每种L3基因片断都有2772个核苷酸,包含一个开放读框(ORF)。这个开放读框(ORF)编码由901个氨基酸组成的蛋白质,VP3(分子量103kD),其等电点为6 0。用这5种蓝舌病毒血清型的核苷酸和氨基酸序列进行分子系统学分析发现,BTV-2血清型与BTV-10、11、13和17的距离较远,美国的五种血清型BTV可分为两个不同的类群。

The complete nucleotide sequences of cognate L3 doublestranded RNA (dsRNA) segments of bluetongue virus (BTV) serotypes 2, 11and 13 encoding the major viral inner capsid protein, VP3 were determined Each cognate L3 segment was 2772 nucleotides long and contained an open reading frame (ORF) The ORF encode the 901 amino acid VP3 protein (103 kD) with a calculated isoelectric point of 6 The molecular phylogenetic analysis using the nucleotide and amino acid sequences of L3 cognate gene of the five BTV serotypes indicated that the BTV2 was more distantly related than BTV10, 11, 13 and 17 The five US BTV serotypes were apparently derived from two distinct gene pools