摘要
目的 研究结缔组织生长因子(connectivetissuegrowthfactor,CTGF)mRNA与转化生长因子β1(transform inggrowthfactorβ1,TGFβ1)mRNA在免疫性大鼠肝纤维化中的表达状况及汉丹肝乐对其的影响作用。方法 用猪血清腹腔内注射复制免疫损伤性肝纤维化模型,造模同时给予汉丹肝乐口服作为肝纤维化防治组;免疫攻击12周后处死动物。原位杂交方法检测肝内CTGFmRNA、TGFβ1mRNA表达。结果 12周后模型组大鼠形成典型的肝纤维化,肝内CTGFmRNA与TGFβ1mRNA表达均显著增强,同时二者阳性分布部位相近,表达程度呈正相关性;以汉丹肝乐防治的大鼠,肝纤维化程度明显减轻,CTGFmRNA和TGFβ1mRNA表达的阳性指数下降,且CTGFmRNA表达与组织病理上肝纤维化变化呈正相关性。结论 CTGF与TGFβ1基因的增高表达与肝纤维化形成有密切关系;汉丹肝乐能有效抑制CTGF与TGFβ1基因的表达。通过在转录环节上阻断肝纤维化相关的细胞内信号转导途径,可能是该药物作用的重要分子机制之一。
ObjectiveTo study the expression of CTGFmRNA and TGFβ1mRNA in immunologically induced liver fibrosis in rats and effect of Handanganle on them MethodsMale wistar rats were given an intraperitoneal injection of porcine serum twice a week for twelve weeks. From the starting injection, Handanganle was fed on rats for precaution The animals were killed after the last injection. CTGFmRNA and TGFβ1mRNA were detected in liver sections by in situ hybridization The semi-quantitative results of the gene expression were analysed in contrast with the stage of hepatic fibrosis ResultsTypical liver fibrosis model in rats was shaped after twelve weeks of injection The positive stain of CTGFmRNA and TGFβ1mRNA increased in animals without Handanganle treatment and was distributed in the areas where fibrosis occurred There was obvious correlation between the strength of expression of CTGF and TGFβ1 In rats receiving Handanganle, the scores of CTGFmRNA decreased as the hepatic fibrosis mildened, the level of TGFβ1mRNA also declined ConclusionThe expression increase of CTGFmRNA and TGFβ1mRNA is correlated closely with hepatic fibrosis. Handanganle can effectively prevent increases in the gene expression of CTGF and TGFβ1One of the mechanisms of the intervention may be due to its blocking the intracellular signalling pathways involved in liver fibrogenesis
出处
《贵州科学》
2003年第1期111-114,共4页
Guizhou Science
基金
贵州省"九五"攻关项目(1996-1028)
