鼠疫耶尔森菌疫苗株荚膜抗原的纯化

Purification of capsular antigen of vaccine Yersinia pestis strain

利用AKTA purifier 100纯化系统纯化盐析法制备的粗制鼠疫荚膜抗原,并将纯化洗脱的蛋白进行透析、浓缩后采用UltrospecTM8000紫外光/可见光分光光度计测定其纯度、蛋白浓度和RIHA试验和胶体金纸色谱法对其主要成份的鉴定。结果发现,粗制鼠疫荚膜抗原经过蛋白纯化仪SuperdexTM凝胶层析柱获得主要组份是种小分子蛋白,其纯度为88.5%,蛋白浓度为1.594 mg/mL,而且RIHA试验证实此蛋白是鼠疫菌F1抗原。

Capsular antigen was extracted and purified from salt fractionation of vaccine Yersinia pestis strain with the AKTA purifier 100 biological macromolecule purified system. Its purity was dialyzed、concentrated 、measured protein purity、its concentration through UltrospecTM8000 Uv /visible spectrophotometer and identified by reverse indirect hemagglutination assay( RIHA). The results showed that the unshaped capsular antigen purified with the purified system regains a kind of small molecular protein. Its purity exceeds 88. 5%,its protein concentration was 1. 594 mg /ml. Application of the RIHA technology of Yersinia pestis confirmed that the purified protein was F1 antigen.