摘要
目的 :对比分析在Logit-log4P和Spline定标方式下检测Apo A1、Apo B结果的差异性。方法 :分别用Logitlog4P和Spline定标方式检测已知浓度的参考物质,再用其检测38份血清样品中Apo A1的浓度(浓度范围40-220mg/dl)及40份血清样品中Apo B的浓度(浓度范围20-220mg/dl),最后对用两种定标方式进行Apo A1、Apo B检测的结果进行对比分析。结果 :采用Logit-log4P和Spline的定标方式进行Apo A1、Apo B检测的结果均具有较高的正确度。用这两种定标方式进行Apo A1检测的结果相比较差异不显著,无统计学意义(P>0.05)。与采用Spline的定标方式相比,采用Logit-log4P的定标方式检测Apo B的指标较低,差异显著,有统计学意义(P<0.05)。根据用这两种定标方式进行Apo A1检测的结果计算其回归方程和相关系数,结果分别为:Yspline=1.100XLogit-log4P-10.10,r=0.997。根据用这两种定标方式进行Apo B检测的结果计算其回归方程和相关系数,结果分别为:Yspline=0.994XLogit-log4P+2.556,r=0.998。结论 :采用Logitlog4P和Spline的定标方式均可准确地检测Apo A1、Apo B的指标。在检测低浓度和高浓度Apo A1的血清样本时,用Logitlog4P和Spline的定标方式测定Apo A1的结果有显著的差异性。在检测中间浓度Apo A1的血清样本时,用Logit-log4P和Spline的定标方式测定Apo A1的结果无明显的差异性。在检测低浓度和中间浓度Apo B的血清样本时,用Logit-log4P和Spline的定标方式测定Apo B的结果有显著的差异性。在检测高浓度Apo B的血清样本时,用Logit-log4P和Spline的定标方式测定Apo B的结果无明显的差异性。用这两种定标方式进行Apo A1、Apo B检测的结果具有较好的相关性。
Objective To compare the effects of Logit-log4P and Spline function calibration methods on Determination of ApoA1, ApoB. Methods Determination of Logit-log4P function and Spline function calibration methods for known concentration of reference material , make accuracy analysis of two calibration methods; Determination of ApoA1 of 38 serum samples (range 40-220mg/dl), ApoB of 40 serum samples (range20-220mg/dl), make comparison analysis and correlation analysis between the two calibration methods. Results For ApoA1, samples are divided into 3 groups according to the concentration: 40-100 mg/dl,100-150 mg/dl, 150-220 mg/dl, Logit-log4P calibration results were as follows: 71.86 ± 17.15,121.38 ± 14.11,161.88 ± 21.47, Spline calibration results were as follows: 69.57 ± 16.40,121.56 ± 17.02,170.63 ± 22.92, for each group results ,analysis by t test, the P values were as follows: 0.001,0.845,0.000, the overall analysis of 38 samples by t test, the P value was 0.200. For ApoB, samples were divided into 3 groups according to the concentration: 20-80mg/dl, 80-140mg/dl, 140-220mg/dl, Logit-log4P calibration results were as follows: 47 ± 15.75,105.32 ± 19.84,174.33 ± 23.86, Spline calibration results were as follows: 48.47 ± 16.91,108.05 ± 18.94,175.00 ± 21.76, for each group results ,analysis by t test, the P values were as follows:0.019,0.003,0.666, the overall analysis of 40 samples by t test, the P value was 0.000. Conclusion Two calibration methods have better accuracy. Determination of serum samples on different concentration gradient: ApoA1, at low and high concentrations, there was statistical difference between two methods (P<0.05), at intermediate concentration, two methods showed no statistical difference (P>0.05) ,the overall analysis of 38 samples, two methods showed no statistical difference (P>0.05). ApoB, at low and intermediate concentrations, there was statistical difference between two methods (P<0.05), at high concentrations, two methods showed no statistical difference (P>0.05) , the overall analysis of 40 samples, there was statistical difference between two methods (P<0.05). Results of Logit-log4P were generally lower than that of Spline calibration method.
