蔬菜中有机磷农药残留的两种酶抑制快速检测方法的比较研究

Comparison of Inhibiting Ache and Plant-esterase Activity for Organophosphorus Pesticide Residues in Vegetables

本实验比较了快速测定蔬菜中有机磷农残的两种酶抑制法即胆碱酯酶抑制法和植物酯酶抑制法,通过正交实验优化了两种酶抑制法的测定条件。对实际蔬菜样品的磷农残测定结果表明:这两种酶抑制快速测定方法均可用于蔬菜中有机磷农残的测定,其加标回收率都达到85%以上。但植物酯酶法测量精密度较好(3%以下),酶源易得,提取、保存方便,在有机磷的快速测定中有更广泛的应用价值。

Based on the toxicology theory that the organophosphorus pesticide can inhibit AChE and plant-esterase activity,two rapid methods of determining organphosphorus pesticide residues in vegetables by inhibiting AchE or plant-esteraseactivity were established. Results showed that the recoveries of adding standards by the two methods were more than 85%, therelative standard deviations (RSD%) was less than 3% for the latter, and the lowest detection limits ranged from 0.01 to 0.6mg/kg for the both methods. The rapid determining methods can be used for the quantification of organophosphorus pesticideresidues in vegetables, especially by the plant-esterase.