流动注射化学发光法测定食品营养添加剂中铅

Determination of Lead in Caleium Propionate by Flow Injection Chemiluminescence

研究了表面活性剂对痕量Pb(Ⅱ)催化H2O2氧化鲁米诺—邻菲啰啉化学发光反应的影响酝挛拢?0为增敏剂,建立了增敏反应化学发光测定痕量铅的新体系。本方法线性范围(1×10-6～1×10-4mg/ml)宽,检出限(2.5×10-7mg/ml)低, pH范围拓宽由原来的3改变成3～8。可用于食品营养添加剂——丙酸钙、乳酸钙、醋酸钙和卵黄高磷蛋白中铅的测定,结果良好。

The effect of surfactant on the chemiluminescence(CL) that Pb(II) catalyses luminal-1,10-phen-H2O2 is studied.Based on Tween-20, a new CL method for determining trace Pb(II) is proposed. there is a linear relationship between the intensityof CL and concentration of Pb(II) in the range of 1×10-6～1×10-4mg/ml; the detection limit of method is 2.5×10-7mg/ml forPb(II);the RSD is 1.9%.The method has been applied to the determination of lead in Calcium propionate with satisfactory results.