Fas配体基因导入对大鼠颈动脉损伤后新生内膜的影响

Effects of adenoviral vector-mediated Fas ligand gene introduction on neointima after rat carotid injury

目的 :探讨腺病毒载体介导 Fas配体 (Fas L)基因导入对大鼠颈动脉损伤后新生内膜的影响。方法 :利用重组腺病毒载体将 Fas L 导入大鼠颈动脉球囊损伤的内膜 ,14d后观察其对新生内膜形成的影响。结果 :通过腺病毒载体介导导入 Fas L 基因的被球囊损伤的大鼠颈动脉 ,与 Ad- β gal基因的对照组相比较 ,14d后损伤血管新生内膜 /中膜面积比降低了 73%(P<0 .0 1)。结论 :Fas L 可抑制新生内膜的增生。

AIM: To study the effects of Fas ligand(FasL) gene transfer mediated by adenoviral vector on neointimal hyperplasia after rat carotid injury. METHODS: Restenosis model was established in SD rat and FasL gene was transduced into the carotid artery by adenoviral vector after carotid balloon injury and neointimal/media area ratio in local artery 14 days after gene tranafer was measured. RESULTS: FasL gene was successfully transducted into the carotid artery after balloon injury. Neointimal/media area ratio was reduced 73% (P<0.01) compared with the control group β gal 14 days after gene transfer. CONCLUSION: FasL gene transfer to the vessel wall inhibits neointimal formation. It is an effective gene therapy to restenosis.