急性肺损伤肺组织IκB激酶-βmRNA的表达及意义

Expression and Significance of IκB kinase-β mRNA in the Lung Tissues of Rabbits Following Hemorrhagic Shock

目的 探讨IκB激酶 -β(IκK -β)在失血性休克继发急性肺损伤 (ALI)中的变化及意义。 方法 采用原位杂交、免疫组化、酶联免疫吸附试验分别检测家兔动物休克前后肺组织IκK -β、核因子 (NF) -κB的表达及外周血肿瘤坏死因子 -α(TNF -α)的含量。并进行肺组织病理学光镜检查。结果 模型组上述指标 [IκK -β( 0 1685± 0 0 164 )、NF -κB( 0 14 69± 0 0 0 83 )、TNF -α( 63 6 72±10 0 2 3 )ng/L]较对照组 [IκK -β( 0 0 42 7± 0 0 2 41)、NF -κB( 0 0 3 5 8± 0 0 0 48)、TNF -α( 199 5 1± 3 5 69)ng/L]比较 ,显著升高 ( P均 <0 0 1) ,肺组织明显病理损害 ;结论 IκK -β激活介导NF

Objective To investigate the changes and significance of IκB kinase-β(IκK-β) in the lung tissues of rabbits with hemorrhagic shock.Methods The expressions of IκK-β and NF-κB in the lung tissues and the concentration of tumor necrosis factor-α(TNF-α) in the plasma were measured by in situ hybridization(ISH),immunohitochemistry and enzyme linked immune adsorbent analysis(ELISA), respectively. And the pathological changes were examined with light microscope in lung tissues.Results In hemorrhagic shock group,the expressions of IκK-β(0 1685±0 0164)and NF-κB( 0 1469±0 0083)in lung tissues , the level of TNF-α(636 72±100 23) in the plasma were obviously higher than those of normal group [IκK-β(0 0427±0 0241),NF-κB(0 0358±0 0048),TNF-α(199 51±35 69)ng/L](all P<0 01),and the severity of organ damages in lung increased following hemorrhagic shock.Conclusions The IκK-β expression , NF-κB activation and cytokine secretion may play an important role in molecular pathogenesis mechanism of acute lung injury following hemorrhagic shock.