高效毛细管电泳法测定清经胶囊中小檗碱含量

HPCE Determination of Berberine in Qingjing Capsules

目的:采用高效毛细管电泳法(HPCE)的毛细管区带电泳分离模式(CZE)分离测定清经胶囊中小檗碱含量并与HPLC法比较。方法:熔融石英毛细管柱:58.5cm (有效长度50cm)×75μm;运行缓冲液:0.2mol·L-1乙酸钠溶液-甲醇(8:2);压力进样:20kPa·s;运行电压15kV;柱上检测:λ=265nm;毛细管柱温:25℃。结果:盐酸小檗碱进样浓度在9.95—159.2mg·L-1范围内线性关系良好(r=0.9999),平均加样回收率为100.4％(RSD=1.9％,n=5)。结沦:该法与HPLC法所测结果吻合,但具有有机溶剂消耗少等优点,可用于清经胶囊中小檗碱含量的质量控制。

Objective: To establish method for determination of berberine in Qingjing capsules by HPCE. Method: Capillary zone electrophoresis (CZE) separation, fused-silica capillary column: 58. 5cm (efficiency length 50cm)×75μm, running buffer: 0.2mol·L-1 sodium acetate solution-methanol (8:2); injection: 20kPa·s; detection wavelength: 265nm; column temperature:25℃. Results: The calibration curve of berberine hydrochloride was linear in the range of 9.95-159.2mg·L-1(r=0. 9999). The average recovery in sample was 100.4%(RSD=1.9%,n=5). Conclusion: This method is accurate as same as HPLC, and could be used for quality control of berberine from Qingjing capsules.