活动期溃疡性结肠炎发病机制的免疫学探讨

IMMUNOPATHOGENESIS STUDY OF PATIENTS WITH ACTIVE ULCERATIVE COLITIS

目的 :目前研究认为溃疡性结肠炎发病机制与免疫因素有关 ,已有研究表明溃疡性结肠炎与白细胞介素6 (IL - 6 )、白细胞介素 8(IL - 8)密切相关。另有报导认为一氧化氮合酶 (NOS)与溃疡性结肠炎有关 ,但是IL- 6、IL - 8与NOS关系的报导并不多见。为了揭示活动期溃疡性结肠炎结肠粘膜细胞因子 (白细胞介素 6、白细胞介素 8)的表达 ,以及与诱导型一氧化氮合酶 (iNOS)的关系 ,进一步探讨溃疡性结肠炎免疫学发病机制 ,设计本研究。方法 :对 34例活动期溃疡性结肠炎粘膜标本及 10例正常结肠粘膜标本进行iNOS的免疫组织化学分析及IL - 6mRNA ,IL - 8mRNA的原位杂交技术检测分析。结果 :34例活动期溃疡性结肠炎粘膜标本IL - 6mRNA有 2 8例阳性 (83% ) ,较对照组明显增高 (P <0 .0 1) ,IL - 8mRNA有 2 7例阳性 (79% ) ,与对照组相比明显增高 (P <0 .0 1) ,iNOS阳性 30例 (88% ) ,与对照组比较增高 (P <0 .0 5 )。结论 :细胞因子 (IL- 6 ,IL - 8)及诱导型一氧化氮合酶 (iNOS)在活动期溃疡性结肠炎免疫学发病机制中起重要作用 ,细胞因子是iNOS活性增加的重要原因。

Objective:Ulcerative colitis (UC) is an inflammatory disease of unknown etiology.Immune factors have been implicated in its pathogenesis. It had been reported that expressions of IL-6,IL-8mRNA as well as NOS in the intestinal mueosal were increased in patients with UC. To investigate the changes and relations of colonic mucosal cells among inducible nitric oxide synthase (iNOS), interleukm 6(IL-6), interleukm 8(IL-8) in patients with active Ulcerative Colitis(UC) as well as to further immunopathgenesis of UC.Methods: Tissue samples from 34 cases and 10 health controls were treated with immunohistochemistry and in situ hybridization.Results:Expressions of IL-6 and IL-8 mRNA in the intestinal mucosal were significantly increased in patients with active UC as compared with health controls ( P< 0.01).with 28(83%) and 27(19%) positive cases respectively.30 cases expressed iNOS,increased rapidcy with that of contral group( P< 0.05).Conclusions:The excessive production of NO reduced by inerleukins is responsible for the hyperdynamic circulation in patients with UC. Interleukins play a role in immunopathgenesis of UC.